| Objective To investigate the effect of different concentrations of isoniazid (INH) and rifampicin (RFP) on cell proliferation, alkaline phosphatase (ALP) activity, expression of typeⅠcollagen and mineralization of osteoblasts derived from newly born SD rat in vitro．Methods Osteoblasts derived from newly born (<24 h) SD rats' calvarias were cultured with INH and rifampicin at different concentrations of 10+3 μg/mL, 20+6μg/mL, 30+12μg/mL, 40+24μg/mL, 50+48μg/mL for some days, then evaluated for cell proliferation, ALP activity, expression of typeⅠcollagen and mineralization using CCK-8, ALP activity assay, immunofluorescence staining and alizarin red staining assay. Results When the concentration of INH and RFP reached 20+6 μg/mL and above, CCK-8 values, ALP activity, type I collagen synthesis and mineralization of OB were significantly decreased compared with those of the control group (P<0.05). With the increase in the concentration of INH and RFP, the inhibition increased gradually. Conclusion Our study shows that INH and RFP at lower concentration could inhibit osteoblasts. After the removal of bone and joint tuberculosis, it is necessary to strictly control the concentration of INH and RFP. |