| Objective To investigate the effects of musk on the proliferation and migration of exogenous bone marrow mesenchymal stem cells (BMSCs). Methods Sixty SD rats were randomly divided into high, medium and low dose musk group and blank control group, and the control serum and musk containing serum were prepared. BMSCs were isolated from fifteen SD rats by whole bone marrow adherence method, which was then cultured to P3 generation. BMSCs were identified by morphological observation, phenotypic identification, and osteogenic and adipogenic induction. After identifying the success of cell culture, BMSCs were intervened by musk containing serum, then the cell increment rate was detected, and Transwell assay was used to detect the effect of musk serum on the migration of BMSCs. Results Exogenous rat BMSCs showed fusiform adherent growth, with good growth state. Phenotypic identification: the expression of CD45 and CD34 were negative, the expression of CD44 and CD90 were positive. After osteogenic and adipogenic induction, the cells could be induced into osteogenic and adipogenic differentiation. Compared with the control group, musk groups of different concentrations could increase the proliferation rate of BMSCs (P<0.05); compared with the control group, different concentrations of musk increased BMSCs migration at 24 h, 48 h, 72 h (P<0.05), with the best effect in the low concentration group. Conclusion Musk serum can promote the proliferation of BMSCs and promote the migration of BMSCs in vitro. |