中国骨质疏松杂志

补肾壮骨方对去卵巢大鼠骨结构和骨代谢作用的研究

Effects of Bushen Zhuanggu compound on bone structure and metabolism in ovariectomized rats

DOI：10.3969/j.issn.1006-7108.2019.02.005

英文关键词:Bushen Zhuanggu compound osteoporosis calcium and phosphorus metabolism bone metabolism bone structure cathepsin K

基金项目:国家自然科学基金面上项目（81874373）；北京市自然科学基金项目（7172126）

作者	单位
陈贝贝郭鱼波刘海霞朱如愿柳辰玥贾强强高思华张东伟*	北京中医药大学中医学院糖尿病研究中心，北京100029

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中文摘要:

目的研究补肾壮骨方对骨质疏松大鼠骨结构和骨代谢的影响及其可能的作用机制。方法用SD雌性大鼠复制去卵巢骨质疏松模型，然后分别灌服补肾壮骨方水提液高剂量（1.4 g/kg）和低剂量（0.7 g/kg）12周。用生化法测定血清钙（S-Ca）、血清磷（S-P）、尿钙（U-Ca∕Cr），尿磷（U-P/Cr）以及血清中高密度脂蛋白胆固醇（high-density lipoprotein cholesterol，HDL-C）、低密度脂蛋白（low-density lipoprotein，LDL）、总胆固醇（total cholesterol，TC）、甘油三酯（triglyceride，TG）的含量；Elisa法测定I 型胶原氨基端前肽（procollagen I N-terminal propeptide，PINP）、I 型胶原羧基端肽（collagen I carboxyl terminal peptide，CTX-I）、尿脱氧吡啶啉（deoxypyridine，DPD）、骨钙素（osteocalcin，OCN）的含量；用放免法测定血清碱性磷酸酶（alkaline phosphatase，ALP）的活性；用双能X线（DEXA）法、Viva CT 和万能试验机测定骨密度、骨微结构和骨生物力学特性；用Western-blotting法测定骨组织蛋白酶（cathepsin）K表达。结果补肾壮骨方水提液能升高血清中的钙、磷、HDL-C和PINP的含量，降低血清中ALP、OCN和CTX-I，及尿液中的钙、磷、DPD的含量。同时，补肾壮骨方水提液可以升高去卵巢大鼠的股骨的骨密度，改善骨微结构，增加骨强度，降低胫骨Cathepsin K的表达水平。结论补肾壮骨方水提液能够抑制去卵巢大鼠的骨量丢失和骨强度下降，改善去卵巢大鼠的骨代谢，其作用机制可能与抑制Cathepsin K 的表达有关。

英文摘要:

Objective To investigate the anti-osteoporotic effects of Bushen Zhuanggu compound on bone structure and bone metabolism in ovariectomized (OVX) rats. Methods Osteoporotic rats model was established by bilaterally ovariectomy in Sprague-Dawley female rats. Bushen Zhuanggu compound aqueous extract (0.7 and 1.4 g∕kg) were orally given to OVX rats for 12 weeks. Biochemical method was used to determine serum levels of calcium (S-Ca) and phosphorus (S-P), urinary levels of calcium (U-Ca∕Cr) and phosphorus (U-P)∕Cr) as well as serum levels of high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein (LDL), total cholesterol (TC) and triglyceride (TG). ELISA was used to examine collagen I amino terminal peptide (PINP), collagen I carboxyl terminal peptide (CTX-I), osteocalcin (OCN) and urine deoxypyridine (DPD). The activity of serum alkaline phosphatase (ALP) was determined by radioimmunoassay. Western-blotting method was used to measure the expression of cathepsin K in the rat tibia. Bone mineral density, microstructure and biomechanics were determined by dual-energy X-ray (DEXA), VivaCT and bone biomechanical properties. Results: Bushen Zhuanggu compound increased the serum levels of calcium, phosphorus, HDL-C and PINP, decreased the urinary levels of calcium and phosphorus and DPD, and reduced serum levels of ALP, OCN and CTX-I. At the same time, Bushen Zhuanggu Compound could increase the femur bone density, improve bone microstructure, increase bone strength and reduced the expression of tibia cathepsin K in OVX rats. Conclusion: Bushen Zhuanggu Compound could exert anti-osteoporosis effect by increasing bone mineral density and strength and improving bone microstructure through regulation of bone metabolism and mineral content in OVX rats, and this effect might be related to inhibition of the cathepsin K expression in rat tibia.

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