|
| 硫酸钙对小鼠骨髓间充质干细胞分化和迁移的影响 |
| Effects of calcium sulfate on the differentiation and migration of mouse bone marrow mesenchymal stem cell |
| |
| DOI：10.3969/j.issn.1006-7108.2019.04.001 |
| 中文关键词: 硫酸钙 间充质干细胞 迁移 骨再生 |
| 英文关键词:calcium sulfate mesenchymal stem cells (MSCs) migration bone regeneration |
| 基金项目:国家自然科学基金（31501152）； 湖南省卫生计生委科研计划课题（B2017042） |
|
| 摘要点击次数: 1201 |
| 全文下载次数: 409 |
| 中文摘要: |
| 目的 分析不同浓度的硫酸钙（calcium sulfate , CaSO4）对小鼠骨髓间充质干细胞（mesenchymal stem cells , MSCs）分化和迁移的影响，探讨CaSO4促进骨修复的细胞机制。方法 分离小鼠骨髓MSCs，绘制细胞生长曲线，评价不同浓度的CaSO4对细胞生长的影响，用qRT-PCR方法评价CaSO4作用后的成骨相关基因Osterix因子和骨钙素的表达，通过刮痕合拢实验和琼脂糖斑点实验，评估CaSO4对MSCs迁移能力的影响。结果 中浓度（0.8 g/L）和低浓度（0.1 g/L）的CaSO4对细胞生长无明显影响，高浓度（1.5 g/L）的CaSO4抑制了细胞增殖；中浓度的CaSO4在作用了1~4 d后，显著提高了MSCs成骨基因Osterix和骨钙素的表达，也明显促进了细胞迁移；高浓度（1.5 g/L）的CaSO4反而抑制了MSCs分化和迁移。结论 一定浓度的CaSO4能够诱导MSCs成骨分化，使MSCs细胞迁移能力增强；成骨细胞的出现可能是细胞迁移能力提高的原因之一。
关键词： 硫酸钙；间充质干细胞；迁移；骨再生 |
| 英文摘要: |
| Objective To study the effects of different concentrations of calcium sulfate (CaSO4) on the differentiation and migration of bone marrow mesenchymal stem cells (MSCs) of mice, and to discuss the cellular mechanism of CaSO4 in bone repair. Methods Bone marrow MSCs were isolated from mice, and the cell growth curves were drawn to evaluate the effect of different concentrations of CaSO4 on cell growth. After CaSO4 treatment, the gene expression of osterix factor and osteocalcin was evaluated by qRT-PCR. The effect of CaSO4 on the migration of MSCs was evaluated by wound healing assay and agarose spot experiment. Results The medium concentration (0.8 g/L) and low concentration (0.1 g/L) of CaSO4 had no significant effect on cell growth, while high concentration (1.5 g/L) inhibited cell proliferation. CaSO4 of the medium concentration significantly promoted osteogene (osterix and osteocalcin) expression and cell migration after 1 to 4 days. In contrast, the high concentration (1.5 g/L) of CaSO4 inhibited the differentiation and migration of MSCs. Conclusion At certain concentration, CaSO4 induced MSCs osteogenesis differentiation, meanwhile, the migration ability of MSCs cells was enhanced. The appearance of osteoblasts may be one of the reason for the enhancement of cell migration.
Key words: calcium sulfate; mesenchymal stem cells (MSCs); migration; bone regeneration |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |