| Objective Detecting serum Osteoprotegerin (OPG) and 25-hydroxyvitamin D [25 (OH) D] levels and peroxisome proliferator activated receptor gamma (PPAR-γ) mRNA expression in newly diagnosed T2DM patients with different levels of bone mass, in order to explore their role and significance in the development of osteoporosis associated with T2DM. Method 135 cases of T2DM admitted to the Department of Endocrinology of our hospital from February 2017 to January 2018 were included in this analysis, and categorized into three groups according to the bone mass: T2DM with normal bone mass group (group A 45 cases), T2DM with osteopenia group (group B 45 cases) and T2DM with osteoporosis group (group C 45 cases). At the same period, data were collected from age and gender matched healthy individuals who attended physical examination at our hospital’s physical examination center as normal control group (group NC 45 cases). Real Time PCR was used to detect the expression of PPAR-γ mRNA in peripheral blood. Correlation of PPAR-γ mRNA level and other indexes in T2DM patients combined with OP was examined using Pearson correlation analysis. Logistic regression analysis was used to study the influential factors of bone mass in T2DM patients. Results Compared with group B, group A had significantly higher levels of 25(OH)D, PINP and HDL-C, and significant lower levels of PPAR-γ mRNA, OPG, β-CTX, HbA1c and HOMA-IR, the difference was statistically significant (P < 0.05). The expression of PPAR-γ mRNA in peripheral blood negatively correlated with 25(OH)D, PINP and HDL-C; and positively correlated with β-CTX, HbAlc, HOMA-IR and OPG. Logistic regression showed that PPAR-γ mRNA, β-CTX, HbAlc and OPG were independent risk factors for newly diagnosed type 2 diabetic patients with osteoporosis. Conclusion The increased expression of PPAR-γ mRNA in peripheral blood and decreased serum 25 (OH) D levels may involve in the development of diabetes and osteoporosis. |