| Objective To detect the effect of aspirin on the proliferation and osteogenic differentiation of adipose derived mesenchymal stem cells of type I osteogenesis imperfecta mice. Methods The adipose derived mesenchymal stem cells (ADSCscol1a1+/-) were isolated and mesenchymal stem cell related marker CD 29, CD 45, et al. were identified by flow cytometry. To validate the multiple differentiation potential of ADSCscol1a1+/-, ALP staining were performed after 7 days of osteogenesis induction, and oil red O staining were performed after 14 days of adipogenic differentiation. The cell proliferation of ADSCscol1a1+/- were assayed by MTS assay after treatment with 0, 0.5, 1, 1.5, 2, 5 and 10 mmol/L aspirin, separately. After osteogenic induction, alkaline phosphatase (ALP) staining and ALP activity were compared among groups, and changes of transcription level of osteogenic differentiation associated genes and signal pathway factors were determined by qPCR. Results ADSCscol1a1+/- have the potential of osteogenesis and adipogenic differentiation. Low and medium concentration (0-2 mmol/L) aspirin promoted ADSCscol1a1+/- proliferation, while high concentration (5-10 mmol/L) aspirin exhibited a repression effect. Compared with control group and osteogenesis group, the ALP level was obviously increased in the osteogenesis+Aspirin group. Moreover, significant up-regulation in transcription level were observed in osteoblastic differentiation associated genes (col1a1, bglap, runx2 and osterix) and β-catenin in Wnt signaling and bmp2 in TGF-β pathway. Conclusion Aspirin promoted the proliferation of ADSCscol1a1+/- and enhanced osteogenic differentiation probably through Wnt and TGF-β pathway. |