| Objective To understand the effect of sulforaphane on the activity of osteoblasts under normoxic conditions, and to explore the rationality of using antioxidants under normoxia. Methods Osteoblasts hfob1.19 were placed under normoxic conditions (20% O2 in the incubator). According to the experimental requirements, sulforaphane was added as 0, 5, 10, 20 μmol/L groups, and cultured for 24 h and 48 h, respectively. Cell proliferation was detected by MTT, apoptosis was detected by flow cytometry, and intracellular ROS was detected. Results In the 24h groups, the OD values ??of 0, 5, 10, 20 μmol/L sulforaphane added groups were 0.63±0.02, 0.77±0.04, 0.91±0.04 and 0.85±0.01, respectively. In the 48h groups, the OD values ??were 0.53±0.04, 0.65±0.04, 0.82±0.02 and 0.76±0.04, respectively. While 5 umol/L and 10 umol/L sulforaphane promoted cell proliferation, 20 umol/L sulforaphane slowed down the growth rate. In the 24h groups, apoptosis rate of 0, 5, 10, 20 μmol/L sulforaphane added groups were 3.76%, 3.71%, 3.38% and 3.33%, respevtively. In the 48h groups, apoptosis rate ??were 4.02%, 3.49%, 3.33% and 3.09%, respectively. It can be seen that sulforaphane could reduce cell apoptosis. The higher the concentration, the more obvious the effect; In the 24h groups, the ROS ??of the cells of 0, 5, 10, 20 μmol/L sulforaphane added groups were 3152.67±118.73, 2762.33±75.25, 2384.67±100.52 and 2165.67±196.02, respectively, and those were 3762.33±74.65, 3291±90.02, 2956.67±78.27 and 2746.33±82.08 for the 48 hours gorup. It can be seen that sulforaphane reduced cellular ROS, and the higher the concentration, the more obvious the decrease. Conclusion Under normoxic conditions, low concentrations of sulforaphane can promote cell proliferation, but the increase of concentration inhibits cell proliferation. The application of sulforaphane can reduce apoptosis and play an antioxidant role. |