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| 芦丁对去卵巢骨质疏松症大鼠的防治作用 |
| Effects of rutin on the prevention and treatment of osteoporosis in ovariectomized rats |
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| DOI：10.3969/j.issn.1006-7108.2019.04.014 |
| 中文关键词: 芦丁 去卵巢大鼠 骨质疏松症 防治 |
| 英文关键词:Rutin ovariectomized rats osteoporosis prevention and treatment |
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| 中文摘要: |
| 目的 探讨芦丁在去卵巢骨质疏松大鼠中发挥的抗骨质疏松作用，找到治疗绝经后骨质疏松症的新思路。方法 取40只大鼠，10只行剖腹手术未摘除卵巢为假手术组，30只行去卵巢手术。去卵巢手术大鼠随机分成3组：OVX组（用生理盐水灌胃）、R5低剂量组[喂食芦丁剂量5 mg (kg·d)]、R10高剂量组[喂食芦丁剂量10 mg (kg·d)]，每日灌胃一次，观察3个月。观察芦丁干预前后大鼠体重和子宫指数的变化，利用双能X线骨密度仪测定大鼠右侧股骨的骨密度(bone mineral density, BMD)。经HE染色分析左侧胫骨组织形态学。用ELISA方法检测IL - 6,IFN-γ、TNF-α的含量，观察芦丁对炎性指标的影响。同时监测干预后的去卵巢大鼠体内E2、1,25(OH)2D3、OCN水平变化。结果 芦丁对去卵巢大鼠体重无显著影响，但干预组子宫指数显著升高，且提高了去卵巢大鼠的BMD，降低了OVX大鼠的炎症因子IL - 6、TNF-α、INF-γ水平。芦丁干预后E2和1,25(OH)2D3水平显著升高，并且通过剂量依赖性逆转了大鼠的血清OCN水平，使骨小梁变厚。此外，芦丁显著改善了去卵巢大鼠骨小梁的平均厚度及平均骨体积分数。结论 芦丁具有明显抗骨质疏松的活性，是一种理想的治疗骨质疏松症的备选药物。 |
| 英文摘要: |
| Objective To explore the anti-osteoporosis effect of rutin in ovariectomized rats and to find a new way to treat postmenopausal osteoporosis. Methods 40 mice were used in this study, among them 10 mice without their ovaries removed by laparotomy were in the sham operation group, and 30 mice underwent ovariectomy. The ovariectomized rats were randomly divided into 3 groups, control group, rutin was graded as low dose group [5 mg (kg·d)] and high dose group [10 mg (kg·d)], once a day, and the control group received normal saline; the observation period was for 3 months. The changes in body weight and uterus index were observed before and after the intervention of rutin. The histomorphology of the left tibia was analyzed by HE staining. The content of IL-6, IFN-γ and TNF-α in was determined by ELISA, and the effect of rutin on inflammatory markers was observed. Changes in E2, 1,25(OH)2D3 and OCN levels in ovariectomized rats after intervention were also monitored. Results The weight of ovariectomized rats was not significantly affected by rutin, but the uterine index was significantly increased in the rutin intervention group. Rutin significantly increased BMD in ovariectomized rats, and decreased the levels of inflammatory cytokines IL-6, IFN-γ and TNF-α in OVX rats. E2 and 1,25(OH)2D3 levels were significantly increased after the rutin intervention, and dose-dependently reversed serum OCN levels in rats. Rutin thickened bone trabeculae. In addition, rutin significantly improved the mean thickness and mean bone volume fraction of the trabecular bone in ovariectomized rats. Conclusion Rutin has significant anti-osteoporosis activity and is an ideal candidate drug for the treatment of osteoporosis. |
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