| Objective To study the effects of Xuling jiangu prescription on bone mineral density (BMD), bone microstructure and blood calcium and phosphorus metabolism in ovariectomized osteoporosis model rats. Methods Forty female 6-month-old SD rats were randomly divided into 4 groups: sham operation group, model group, Xuling jiangu prescription group [12.7083 g/(kg·d)] and calcium carbonate group [104.1875 mg/(kg· d) ], the sham operation group only had fat around the ovary removed, and the other group underwent bilateral ovariectomy to establish a model of osteoporosis. After 12 weeks of drug intervention, BMD of the left tibia was detected by dual-energy X-ray absorptiometry. The microstructure of the right tibia was observed by Masson staining. Tartrate resistant acid phosphatase, serum calcium and serum phosphorus were detected by serum biochemistry. Results Compared with the sham group, the BMD of the model group was significantly decreased, the trabecular bone was fractured, sparse and irregularly arranged, the expression of TRAP increased, the serum calcium content decreased and the serum phosphorus content increased. Compared with the model group, the BMD of the Xuling jiangu group increased, the number of trabecular bone increased and the arrangement was regular, the expression of TRAP decreased, the serum calcium content increased, and the blood phosphorus content decreased. Conclusion Xuling jiangu prescription can improve BMD of the tibia of ovariectomized osteoporosis rats and improve the microstructure of the bone tissue. Its mechanism may be related to the regulation of serum calcium and phosphorus metabolism steady state. |