| Objective To investigate the relationship between plasma vitamin D receptor (VDR) mRNA expression and levels of 25(OH)D and triglyceride (TG) in patients with type 2 diabetes mellitus (T2DM) complicated with osteoporosis. Methods We selected 100 patients with newly diagnosed type 2 diabetes who were outpatients or inpatients of the Department of Endocrinology of Affiliated Hospital of Zunyi Medical College from June 2016 to May 2017. According to the results of bone mineral density (BMD), 48 patients were in the T2DM alone group and 52 patients were in the T2DM with osteoporosis group (T2DM+OP group). Ninety healthy controls (NC group) matched by age and gender from the physical examination centre of Zunyi Medical College were selected. The level of serum 25(OH)D was detected by electrochemiluminescence, and TG was detected by an automatic biochemical analyzer. Real-time fluorescence quantitative polymerase chain reaction (Real Time-PCR) was used to detect the expression level of VDR mRNA in peripheral blood. Results Plasma 25(OH)D level decreased in T2DM+OP group compared with T2DM alone group [(8.76±3.21) vs (12.46±4.35) ng/mL, t=32.423, P=0.000], the expression level of VDR mRNA decreased [(0.35± 0.12) vs (1.07±0.23), t=15.365, P=0.001] and TG level increased [(4.1±1.26) vs (2.98±0.56) mmol/L, t=11.765, P=0.000]. Pearson correlation analysis results showed that the plasma levels of VDR mRNA expression positively correlated with 25 (OH) D levels (correlation coefficient r = 0.717, P<0.05), and HbAlc, HOMA-IR, Fins, FPG and 2 HPG negatively correlated with TG (correlation coefficient r was -0.342, -0.353, -0.361, -0.546, -0.426 and -0.342, respectively; P<0.05). Conclusion The expression of VDR mRNA, the decrease of 25(OH)D levels and the increase of TG levels in peripheral blood may involve in the occurrence and development of T2DM and osteoporosis. |