| Objective To investigate the effects of whole body vibration training combined with sinusoidal alternating electromagnetic field on bone mineral density, bone metabolism and bone biomechanical properties in ovariectomized osteoporotic rats. Methods One hundred rats were randomly divided into the ovariectomized group and the control group. Ovariectomy and sham operation were performed respectively. The rats were recovered for 6 weeks. After recovery, the successfully ovariectomized rats were randomly divided into model groups (MO group), whole body vibration training group (W group), sinusoidal alternating electromagnetic field group (S group) and whole body vibration training + sinusoidal alternating electromagnetic field group (WS group), and the control group was classified as sham operation group (SO group). The intervention was performed for 16 weeks. After the intervention, the rats were tested for bone mineral density, bone metabolism and bone biomechanical properties. Results After 16 weeks of intervention, the body weight of the rats in the MO group and the S group was significantly higher than that in the SO group, W group and WS group (P<0.05); bone density index and serum estradiol concentration in the W group, WS group and SO group were significantly higher than that of the MO group (P<0.05). The serum concentration of estradiol in the SO group was significantly higher than that in the S group, W group and WS group (P<0.05). Serum concentration of OC and ALP in the M group was significantly higher than that of SO group, W group, S group and WS group (P<0.05). For urinary DPD/Cre, Ca/Cre and P/Cre concentration, M group rats had significantly higher levels than rats in SO group, W group, S group and WS group (P<0.05). The maximal femoral load and elasticity model of rats in SO group, W group, S group and WS group were significantly higher than those in MO group (P<0.05). There was no significant difference in femoral fracture load between SO group, W group, S group and WS group (P>0.05). The femoral elastic modulus of SO group, W group, S group and WS group was significantly higher than that of MO group (P<0.05). The femoral elastic modulus of the rats in the SO group was significantly higher than that in the S group (P<0.05), but was not significantly different from that in the W group and the WS group (P＞0.05). In the L4 vertebral compression test, the maximal femoral load and elasticity model of SO group, W group, S group and WS group were significantly higher than those of MO group (P<0.05); rats in the SO group had significantly higher values than those in the S group and the W group (P<0.05), but was not significantly different from rats in the WS group (P＞0.05). Conclusion Whole body vibration training, sinusoidal alternating electromagnetic field, whole body vibration training combined with sinusoidal alternating electromagnetic field can be applied to ovariectomized osteoporotic rats to increase bone density, inhibit bone resorption, balance bone metabolism, and improve bone structure and mechanical properties of materials. Whole body vibration training combined with sinusoidal alternating electromagnetic field energy is better than the simple use of whole body vibration training or sinusoidal alternating electromagnetic field, thus has certain promotion value in clinical application. |