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| miR-187-5p 对骨髓间充质干细胞成骨分化能力的调控作用 |
| Regulative function of miR-187-5p in enhancing the differentiation capacity of bone marrow mesenchymal stem cells into osteoblasts |
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| DOI：10.3969/j.issn.1006-7108.2019.08.012 |
| 中文关键词: 骨质疏松 骨髓间充质干细胞 成骨分化 miR-187-5p |
| 英文关键词:osteoporosis bone marrow mesenchymal stem cells osteogenic differentiation microRNA-187-5p |
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| 中文摘要: |
| 目的 利用小鼠骨髓间充质干细胞（BMSCs）的成骨分化模型，探讨 miR-187-5p 在成骨分化中的表达趋势及调控作用。方法 通过切除雌性小鼠双侧卵巢构建小鼠骨质疏松模型；应用qRT-PCR技术检测组织和细胞中miR-187-5p的表达；应用基因转染技术观察过表达或敲减miR-187-5p对BMSCs向成骨分化的影响；应用茜素红和碱性磷酸酶染色检测BMSCs中矿化结节的数量和矿化区域的染色面积。结果 qRT-PCR结果显示miR-187-5p在骨质疏松模型小鼠的骨组织及BMSCs中均表达下降。过表达miR-187-5p可提高ALP、Collagen-1、Runx2、BMP4、OCN和OPN 等成骨分化相关基因mRNA的表达，促进BMSCs向成骨分化；而敲减miR-187-5p降低ALP等成骨分化相关基因mRNA的表达，抑制BMSCs向成骨分化。在体实验同样证实，过表达miR-187-5p可以显著促进骨质疏松小鼠BMSCs向成骨分化，改善小鼠的骨质疏松表型。结论 过表达miR-187-5p促进BMSCs向成骨分化，敲减miR-187-5p抑制BMSCs向成骨分化。 |
| 英文摘要: |
| Objective Using the osteoblast differentiation model of mouse bone marrow mesenchymal stem cells (BMSCs), the expression trend and regulatory effect of miR-187-5p in osteoblast differentiation were investigated. Methods Osteoporosis model was established by excising the bilateral ovaries of female mice. qRT-PCR was used to detect the expression of miR-187-5p in bone tissues and BMSCs. Gene transfection technique was applied to overexpress or knockdown miR-187-5p. The number of mineralized nodules and the mineralized staining areas in BMSCs were detected by Alizarin red and alkaline phosphatase staining. Results qRT-PCR results showed that, compare to the sham group, the expression of miR-187-5p decreased in bone tissue and BMSCs in OVX group. Overexpression of miR-187-5p effectively increased the mRNA level of osteogenic differentiation-related genes such as ALP, collagen-1, Runx 2, BMP 4, OCN, and OPN, suggesting that the overexpression of miR-187-5p promoted BMSCs to differentiate into osteoblasts. However, the knockdown of miR-187-5p effectively reduced the mRNA level of osteogenic differentiation-related genes, suggesting that the knockdown of miR-187-5p inhibited osteogenic differentiation of BMSCs. In vivo experiments also confirmed that the overexpression of miR-187-5p significantly promoted osteogenic differentiation of BMSCs in osteoporotic mice and then improved the osteoporotic phenotype of mice. Conclusion Overexpression of miR-187-5p promotes BMSCs osteoblast differentiation, and miR-187-5p knockdown inhibits BMSCs osteoblast differentiation. |
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