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| 骨疏康颗粒对去卵巢小鼠体重、血清骨代谢指标和组织形态学的影响 |
| Effect of Gushukang granule on body weight, serum bone metabolic parameters, and histomorphometry in ovariectomized mice |
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| DOI：10.3969/j.issn.1006.7108.2019.09.003 |
| 中文关键词: 中医中药 骨疏康颗粒 药理学 去卵巢小鼠 骨吸收 骨生成 |
| 英文关键词:Chinese traditional medicine Gushukang granules pharmacology ovariectomized mice bone resorption bone formation |
| 基金项目:国家科技部重点领 “创新团队”计划项目（2015RA4002）；国家教育部“创新团队”发展计划项目（IRT1270）；国家自然科学基金重点项目（81730107）；国家自然科学基金项目(81673991,81603643) |
| 作者 | 单位 | | 杨骏杰1,2,3 赵永见1,2,3 王强1,2,3 张岩1,2,3 李晨光1,2,3 施杞1,2,3 王拥军1,2,3,4 舒冰1,2,3
赵东峰 ,2,3* | 1.上海中医药大学附属龙华医院，上海 200032
2.上海中医药大学脊柱病研究所，上海 200032
3.教育部筋骨理论与治法重点实验室，上海 200032
4.上海中医药大学康复医学院，上海 201210 |
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| 中文摘要: |
| 目的 通过检测骨疏康颗粒（GSK）对去卵巢(OVX)小鼠的体重、血清骨代谢指标和组织形态学的作用，进一步阐述GSK延缓OVX小鼠骨量丢失的药理学作用。方法 3月龄雌性C57BL/6J小鼠去卵巢造模后，一周内给予GSK灌胃3个月：低[2g/(kg?d)]、中[4g/(kg?d)]、高[8g/(kg?d)]。干预期间，分别在1、2、3月内，称量各组小鼠体重。实验结束后，采集各组小鼠血液检测骨吸收β胶原降解产物（β-CTX）的水平和骨生成指标：I型前胶原氨基端肽（PINP）、血清中碱性磷酸酶（ALP）、骨钙素（OCN）的水平；同时，阿尔新蓝染色观察腰椎骨量变化。结果 GSK干预1、2、3个月后，小鼠体重未见明显的变化。GSK干预3月后小鼠血清中β-CTX的表达水平下降；骨生成指标PINP、ALP和OCN水平升高。GSK干预后OVX小鼠腰椎的骨量增加、腰椎结构相关参数优化。结论 GSK同时调控OVX小鼠的骨吸收和骨生成指标的表达，延缓OVX诱导的骨量丢失，提高OVX小鼠的骨量。 |
| 英文摘要: |
| Objective By examining the effect of Gushukang granule (GSK) on weight, serum, and histological morphology of ovariectomized (OVX) mice, the pharmacological effect of GSK on delaying bone loss in OVX mice was further elaborated. Methods After OVX, GSK granules were administrated intragastrically to 3-month-old C57BL/6J mice for 3 months, with low dose (2g/kg?d), middle dose (4g/kg?d), or high dose (8g/kg?d) of GSK. Body weight was measured at 1-, 2-, and 3-month, respectively. At the end of the intervention, the expression of precollagen amino terminal peptide (PINPX), alkaline phosphatase (ALP), OCN, and β-CTX and were measured. At the same time, the changes of bone mass of the lumbar vertebrae were observed with Alcian blue staining. Results GSK granules exerted no significant effect on the body weight at the 1-, 2-, and 3- month of intervention. After 3 months of GSK intervention, the expression of β-CTX decreased, but the levels of bone formation markers PINP, ALP, and OCN increased. After GSK intervention, bone mass of the lumbar spine increased in OVX mice. and the bone structure-related parameters were optimized in the lumbar vertebrae. Conclusion GSK regulates the expression of bone resorption and bone formation parameters in OVX mice, delays the bone loss induced by OVX, and increases the bone mass in OVX mice. |
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