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| 质子磁共振波谱测定大鼠椎体脂肪含量的可行性研究 |
| The feasibility study of measuring the fat content in vertebral body by proton magnetic resonance spectroscopy in rats |
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| DOI：10.3969/j.issn.1006-7108.2019.10.002 |
| 中文关键词: 骨髓 质子磁共振波谱 脂肪分数 骨质疏松 脂肪酸 大鼠 |
| 英文关键词:bone marrow proton magnetic resonance spectroscopy fat fraction osteoporosis fatty acid rat |
| 基金项目:国家自然科学基金（81871325）；上海市卫生和计划生育委员会面上项目（201640092）；上海申康医院发展中心项目（SHDC22015026，16CR4029A）；中央高校基本科研业务费专项资金资助项目（22120180380）；上海市科委医学引导类（中、西医）科技支撑项目（19411965300） |
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| 中文摘要: |
| 目的 探讨临床商用3.0 T磁共振扫描仪对大鼠椎体行质子磁共振波谱（proton magnetic resonance spectroscopy，1H-MRS）检查评估骨髓脂肪含量的可行性。方法 15只3月龄正常雌性SD大鼠以及5只骨质疏松模型SD大鼠（去卵巢法）行多体素点分辨波谱法检查测定腰5椎体脂肪分数（fat fraction，FF），分析两名放射科医师测量正常大鼠腰5椎体FF值的一致性，并观察正常和骨质疏松模型大鼠的波谱以及病理特点。结果 两名放射科医师测得的正常大鼠腰5椎体FF值分别为(10.92±3.31)%、(11.10±3.20)%，组内相关系数为0.954。1H-MRS显示水峰明显高于饱和脂肪酸主峰（长链亚甲基质子），而不饱和脂肪酸峰烯烃质子和亚甲基质子（与烯烃质子相连）未见明确显示，HE染色显示骨髓内脂肪细胞少见，散在分布，体积较小；骨质疏松模型大鼠腰5椎体的FF值为(20.13±4.20)%，1H-MRS显示水峰明显高于饱和脂肪酸主峰（长链亚甲基质子），但饱和脂肪酸主峰有所升高，不饱和脂肪酸峰烯烃质子和亚甲基质子（与烯烃质子相连）明确显示，信号振幅较小，HE染色显示骨髓内脂肪细胞增多，弥漫分布，体积增大。结论 临床商用3.0 T磁共振扫描仪行1H-MRS检查测定FF值评估大鼠椎体骨髓脂肪含量具备可行性。 |
| 英文摘要: |
| Objective To study the feasibility of proton magnetic resonance spectroscopy (1H-MRS) performed by clinical 3.0 T magnetic resonance imaging (MRI) scanner to measure the fat content in vertebral body of rats. Methods Fifteen healthy female Sprague-Dawley rats aged 3 months and 5 osteoporosis (OP) rats after bilateral ovariectomy underwent multivoxel point-resolved spectroscopy to measure the fat fraction (FF) in vertebral body of lumbar 5 (L5). The consistency of the FF values of L5 vertebral bodies in healthy rats measured by two radiologists was analysed, and the features of 1H-MRS and pathology of the healthy and OP vertebral bodies were observed. Results The FF values of L5 vertebral bodies in the healthy rats measured by two radiologists were 10.92%±3.31% and 11.10%±3.20%, respectively, and the intra-class correlation coefficient was 0.954. In the bone marrow of the healthy group, 1H-MRS showed that water peak was significantly higher than the dominant peak of saturated lipid (bulk methylene protons). But the peaks of unsaturated lipids (olefinic protons and methylene protons) were not obvious. HE staining showed that adipocytes were rare, scattered, and small. The FF value of L5 vertebral bodies in the OP rats was 20.13%±4.20%. In the bone marrow of the OP group, 1H-MRS showed that water peak was also significantly higher than the dominant peak of saturated lipid, but the latter was increased. The peaks of unsaturated lipids (olefinic protons and methylene protons) were obvious, but their amplitudes were weak, and HE staining showed that adipocytes were increased, diffuse, and large. Conclusion The measurement of FF using 1H-MRS performed by clinical 3.0 T MRI scanner is a feasible way to monitor the fat content of rat vertebral body. |
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