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| 低钙饲料联合地塞米松致大鼠骨质疏松模型的建立 |
| Establishment of a rat model of osteoporosis induced by low calcium diet combined with dexamethasone |
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| DOI：10.3969/j.issn.1006-7108.2019.10.006 |
| 中文关键词: 骨质疏松 地塞米松 低钙饲料 骨密度 |
| 英文关键词:osteoporosis dexamethasone low calcium feed bone mineral density |
| 基金项目:国家重点研发计划资助（2018YFC1706601);吉林省 医药产业发展引导资金项目（20150311048YY) |
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| 中文摘要: |
| 目的 探讨通过低钙饲料联合肌肉注射地塞米松的方法建立大鼠骨质疏松模型的可行性。方法 将20只3月龄雌性 SD大鼠随机分为正常对照组（正常饲料+牛理盐水）和模型组（低钙饲料+地塞米松），每组10只大鼠。模型组每3天注射一次地塞米松，按照1 mg/kg的给药剂量在大鼠的腿部肌肉进行注射，空白组每3天注射一次等量的生理盐水，持续6周。分别在给药4周后通过双能X线吸收骨密度测量仪对大鼠进行骨密度检测，第6周时再次对大鼠的骨密度进行测量，并对其骨灰重/干重、骨钙、骨磷、血钙、血磷、骨组织形态学等指标进行进一步的说明。结果 第4周模型组的骨密度与空白组比较，差异无统计学意义（P>0.05),第6周模型组的骨密度显著低于空白组（P<0. 01)，第6周模型组骨组织中的灰重/干重显著低于空白组（P<0.01)，第6周模型组的骨钙和骨磷含量均显著低于空白组（P<0.01)，第6周模型组血清中的钙浓度著高于空白组（P<0.01),第6周模型组血磷的浓度显著低于空白组（P<0.01),从第6周的病理组织切片中可明显看出模型组的骨小梁不完整,且存在大量脂肪细胞。结论 低钙饲料联合地塞米松的造模方法切实可行，且能缩短造模时间。 |
| 英文摘要: |
| Objective To study the feasibility of establishing a rat osteoporosis model by low calcium diet combined with intramuscular dexamethasone. Methods 20 female SD rats of 3 months old were randomly divided into two groups ： normal control group (normal feed + normal saline)，model group (low calcium feed + dexamethasone)，with 10 rats in each group. The model group received dexamethasone every three days, as intramuscular injection at the leg at a dose of 1 mg/kg，and the same amount of normal saline was injected into the blank group. Bone mineral density was measured by a dual energy X-absorption bone densitometer after four weeks of administration. Bone mineral density was measured again in the sixth week, and further evaluated by indicators such as ashes/dry weight, bone calcium, bone phosphorus, blood calcium, blood phosphorus, and bone histomorphology. Results There was no significant difference in bone mineral density between the model group and the blank group (P>0. 05) at the forth week. Bone density of the model group at the sixth week was significantly lower than that in the blank group (P<0. 01). The ash weight/dry weight in the bone tissue of the model group was significantly lower than that in the blank group (P<0. 01). The contents of bone calcium and bone phosphorus in the model group were significantly lower than those in the blank group (P<0. 01). The serum calcium concentration in the model group was significantly higher than that in the blank group ( P <0. 01). The concentration of phosphorus of the model group was significantly lower than that of the blank group (尸<0. 01). It was evident from the pathological tissue sections that the trabecular bone of the model group was incomplete and there were a large number of fat cells. Conclusion Dexamethasone + low calcium feed modeling method is feasible and can shorten the model time. |
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