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| 淫羊藿苷对兔股骨MEG3、H19和DANCR表达的影响 |
| Effect of icariin on the expression of lncRNA MEG3，H19 and DANCR in rabbit bone |
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| DOI：10.3969/j.issn.1006-7108.2019.10.015 |
| 中文关键词: 淫羊藿苷；长链非编码RNA 母系表达基因3 H19 DANCR 骨形态发生蛋白2 |
| 英文关键词:icariin lncRNA MEG3 H19 DANCR BMP2 |
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| 中文摘要: |
| 目的 研究淫羊藿苷对去卵巢新西兰白兔不同部位骨中长链非编码RNA母系表达基因3 ( maternally expressed gene 3, MEG3)、H19、DANCR/ANCR( anti-differentiation ncRNA)和骨形态发生蛋白 2 (bone morphogenetic protein 2，BMP2)的表达情况。 方法 选取48只新西兰白兔（7月龄）制作骨质疏松组模型，根据体重随机分为假手术组、去卵巢模型组（OVX)和淫羊藿苷治疗组（OVX加治疗）。淫羊藿苷治疗组于术后第4周开始采用淫羊藿苷[20 mg/(kg ? d)]灌胃给药，去卵巢模型组与假手术组每天给予等量的生理盐水灌胃，持续8周。术后12周通过μCT图像进行三维重建来评价骨质疏松模型的建立以及股骨的BV/TV、Tb?Th、Tb ?N、BS/BV、Tb? Sp等的变化;术后12周时通过qRT-PCR检测股骨、椎骨、颅骨组织中长链非编码RNA MEG3、H19、DANCR的表达水平以及Runx2的mRNA表达水平;通过免疫组化检测股骨石蜡切片中BMP2的表达情况。结果 与去卵巢模型组相比，淫羊藿苷通过降低长链非编码RNA MEG3、H19和DANCR的水平，提高了骨质疏松兔股骨的骨小梁体积，其对椎骨中三种lncRNA的影响表现为使MEG3的表达水平降低了（P<0. 05)，对颅骨的影响集中在DANCR的表达变化方面。接受淫羊藿苷治疗的兔的股骨，BMP2的表达情况最高。结论 研究淫羊藿苷对lncRNA的影响，对骨质疏松的治疗具有指导意义。 |
| 英文摘要: |
| Objective To study the effects of icariin on the expression of long non-coding RNA MEG3 (maternally expressed gene 3)，H19, DANCR/ANCR (anti-differentiation ncRNA) and BMP2 in different parts of the bone of New Zealand white rabbits after ovariectomy. Methods 48 New Zealand white rabbits (age of 7 months) were used to make the model of osteoporosis group，which was randomly divided into 3 groups as sham operation Group，ovariectomized model group ( OVX) and icariin treatment Group (OVX plus treatment) according to body weight. From the 4th week after operation，the treatment group received gastric administration of icariin [ 20 mg/(kg ?d) ]，and the sham and OVX groups received the same amount of saline every day for 8 weeks. Three-dimensional reconstruction of μCT images was performed 12 weeks after operation to evaluate the establishment of osteoporosis model and femur BV/TV, Tb ? Th, Tb ? N BS/BV, Tb ? Sp and other changes. The expression level of long chain non-encoded RNA MEG3, H19 and DANCR and the mRNA expression level of Runx2 in femur, vertebrae and skull tissue and were detected by qRT-PCR at 12 weeks after operation. Immunohistochemistry was used to detect the expression of BMP2 in femoral paraffin section. Results Compared with the ovariectomized model group, icariin reduced the level of long-chain non?coding RNA MEG3, H19 and DANCR to improve the volume of femoral trabeculae in osteoporosis rabbits, while the effect of icariin on the three lncRNAs in the vertebrae was mainly on reducing the expression level of MEG3 (P<0. 05)，and the influence on the skull was mainly on the changes in DANCR expression. The expression of BMP2 in femur treated by icariin was the highest. Conclusion Studying the effect of icariin on lncRNA has guiding significance for the treatment of osteoporosis. |
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