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| 续苓健骨汤含药血清对MC3T3-E1细胞分化及增殖的影响 |
| The effect of Xuling strong bone decoction containing serum on the proliferation and differentiation of MC3T3-E1 cells |
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| DOI：10.3969/j.issn.1006.7108.2019.11.008 |
| 中文关键词: 中医中药 续苓健骨 含药血清 MC3T3-E1细胞 细胞增殖 成骨分化 |
| 英文关键词:traditional Chinese medicine Xuling strong bone decoction drug containing serum MC3T3-E1 proliferation differentiation |
| 基金项目:福建省属科技厅公益类科研院所基本科研专项（2015R1035-11, 2018R1035-15）；福建省卫生系统中青年骨干人才培养项目（2015-ZQN-JC-36） |
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| 中文摘要: |
| 目的 探讨续苓健骨汤含药血清对MC3T3-E1成骨细胞分化及增殖的影响。方法 制备续苓健骨汤含药血清，实验分为空白对照组、含药血清低剂量组、中剂量组和高剂量组。采用CCK-8法和流式细胞术检测续苓健骨汤含药血清对MC3T3-E1细胞增殖和细胞周期的影响；碱性磷酸酶（ALP）活性测定MC3T3-E1细胞的成骨分化能力；茜素红染色检测MC3T3-E1细胞的矿化能力；实时荧光定量PCR检测成骨分化基因Runx2、OC、Bmp2、Col1a1mRNA水平。结果 与空白对照组比较，中、高剂量续苓健骨汤含药血清能促进 MC3T3-E1 细胞增殖、S期细胞比率和细胞增殖指数，并且呈现一定的剂量依赖性；同时中高剂量续苓健骨汤含药血清组能明显提高 MC3T3-E1 细胞ALP活性（P<0.01）和钙化能力（P<0.01），促进 Runx2、OC、Bmp2、Col1a1 mRNA的表达（P<0.05）。结论 续苓健骨汤含药血清能促进成骨细胞MC3T3-E1的增殖，并通过上调骨形成相关基因Runx2、OC、BMP2、Col1a1的表达水平，提高MC3T3-E1细胞的成骨能力。 |
| 英文摘要: |
| Objective To study the effect of Xuling strong bone decoction containing serum on the proliferation and differentiation of MC3T3-E1 cells. Methods The serum containing Xuling strong bone decoction medium was prepared. The experiment was divided into blank control group, drug-containing serum low-dose group, medium-dose group, and high-dose group. CCK-8 method and flow cytometry were used to detect the effect on the proliferation and cell cycle of MC3T3-E1 cells. Alkaline phosphatase (ALP) activity was examined to determine the osteogenic differentiation ability of MC3T3-E1 cells. The mineralization of MC3T3-E1 cells was detected with Alizarin red staining. The expression levels of osteogenic differentiation genes Runx2, OC, BMP2, and Col1a1 were detected using real-time fluorescent quantitative PCR. Results Compared with those in the blank control group, the proliferation, S phase cell ratio, and cell proliferation index of MC3T3-E1 cells were promoted in the low-dose and high-dose group in a dose-dependent manner. ALP activity (P<0.01) and calcification ability (P<0.01) were significantly increased in high-dose group. Also, the mRNA expressions of Runx2, OC, BMP2, and Col1a1 were promoted (P<0.05). Conclusion In a certain concentration range, Xuling strong bone decoction containing serum promotes the proliferation and differentiation of MC3T3-E1 osteoblasts. |
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