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| LC-MS法筛选强骨饮对绝经后骨质疏松症患者全血差异蛋白的研究 |
| Analysis of differentially expressed proteins in whole blood in the treatment of postmenopausal osteoporosis with Qianggu decoction by LC-MS |
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| DOI：10.3969/j.issn.1006-7108.2019.12.002 |
| 中文关键词: 液相色谱串联质谱 蛋白质组学 绝经后骨质疏松症 强骨饮 |
| 英文关键词:liquid chromatography tandem mass spectrometry proteomics postmenopausal osteoporosis Qiangguyin |
| 基金项目:国家自然科学基金(8157140353，81873129)；“十三五”浙江省中医药（中西医结合）重点学科建设计划（2017-XK-A16） |
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| 中文摘要: |
| 目的 应用LC-MS法筛选分析经强骨饮治疗的绝经后骨质疏松症患者（postmenopausal osteoporosis，PMOP）和未经治疗的绝经后骨质疏松症患者全血差异蛋白，探索强骨饮治疗绝经后骨质疏松症的作用机制。方法 采用液相色谱-串联质谱联用测定法分别对7例经强骨饮治疗后的绝经后骨质疏松症患者（治疗组）及8例未经治疗的绝经后骨质疏松症患者（对照组）全血蛋白进行检测,并对差异蛋白质进行定量分析,筛选出差异蛋白，进行生物信息学分析。结果 分析治疗组与对照组患者全血筛选出的91个差异蛋白, 以差异倍数大于1.2 倍作为显著上调、小于0.83作为显著下调,确认有 31个蛋白表达显著上调,60个蛋白表达显著下调。对所有差异蛋白进行GO功能注释分析，发现上调的差异蛋白主要位于细胞质内，而有22 %的下调蛋白在细胞外，有 1个细胞骨架蛋白。差异蛋白参与的主要细胞活动为单一生物过程、细胞代谢过程等，分子功能主要为催化活性、分子功能调节剂和结构分子活动等。结论 LC-MS法能有效筛选出强骨饮治疗的PMOP患者和未经治疗的PMOP患者全血中的差异蛋白，强骨饮治疗PMOP的作用机制是一个由多种蛋白质分子参与的结果,其关键的靶标和具体的调控机制尚需进一步的研究探讨。 |
| 英文摘要: |
| Objective To screen and analysis of whole blood differential proteins in postmenopausal osteoporosis patients treated with Qiangguyin and untreated postmenopausal osteoporosis patients by LC-MS, and to explore the mechanism of Qiangguyin in the treatment of postmenopausal osteoporosis. Methods In this study, the whole blood protein was detected by liquid chromatography-tandem mass spectrometry in the treatment group (n=7) and the control group (n=8), and the differential proteins were quantitatively analyzed. Results A total of 91 differential proteins were screened from the whole blood of Qiangguyin treatment group and control group. Among them, 31 proteins were significantly up-regulated and 60 proteins were significantly down-regulated. GO function annotation and functional analysis were performed on all identified proteins, and it was found that the up-regulated differential proteins were mainly located in the cytoplasm, almost all in the cells, while 22% of the down-regulated proteins were outside the cell, 36% were in the cytoplasm, and 1 was cytoskeletal protein. The main activities involved in differential proteins are single biological processes, metabolic processes, biological regulation, and responses to stimuli. The molecular functions are mainly catalytic activity, molecular function regulators and structural molecular activities. Conclusion LC-MS can effectively screen out the differential proteins of Qianggu Decoction in the treatment of PMOP. The mechanism of Qiangguyin in the treatment of postmenopausal osteoporosis is the result of a variety of protein molecules involved, its key targets and specific regulation. The mechanism needs to be further explored and verified. |
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