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| 失神经及制动后兔肱骨骨密度与生物力学性能的对比研究 |
| Comparative study of bone mineral density and biomechanical properties of rabbit humerus after denervation and fixation |
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| DOI：10.3969/j.issn.1006-7108.2019.12.003 |
| 中文关键词: 失神经 制动 骨密度 力学分析 骨质疏松 |
| 英文关键词:denervation brake bone density mechanical analysis osteoporosis |
| 基金项目:国家自然科学基金资助项目（81460198）；广西壮族自治区教育厅科研资助项目(201010LX330) |
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| 中文摘要: |
| 目的 对比分析失神经和制动后兔肱骨生物力学特性及骨密度的改变。方法 将54只新西兰兔随机分成正常组、失神经组和制动组,每组设置4周、8周和12周三个取材时间点。每组18只兔子，每个时间点6只兔子。正常组不做任何处理，失神经组兔子完全离断右侧上肢臂丛神经,制动组采用石膏与石膏托对右上肢进行牢靠的固定。主要检测指标:在造模后4周、8周和12周时取出兔右侧肱骨进行检测，称量肱骨的湿重，三点弯曲试验测定骨力学特性，双能X线骨密度仪检测骨密度，HE染色观察骨组织细微结构。结果 失神经和制动组的骨密度在造模后均低于同时间的正常组（P＜0.05）。失神经组肱骨骨密度与制动组相比虽有所降低，但差异不具有统计学意义（P＞0.05）。失神经组与制动组相比，失神经组的最大负荷、弯曲强度在造模后各个时间点都低于制动组，但只有到第12周时差异才具有显著性（P＜0.05）。失神经和制动组的右侧肱骨湿重随着时间的延长逐渐降低。但失神经组肱骨湿重与制动组相比差异不具有统计学意义（P＞0.05）。与正常组骨组织形态学相比，失神经组和制动组均出现骨小梁变细,小梁之间出现明显断裂，出现大量空泡状结构。结论 失神经和制动均可造成严重的骨质疏松。就两者造成的骨质疏松程度上来说，失神经对骨质力学性能的影响较制动更为严重。力学载荷的降低在导致骨质疏松的过程中起到了更为重要的作用。 |
| 英文摘要: |
| Objective The changes in biomechanical properties and bone mineral density of humerus in rabbits after denervation and braking were compared and analyzed. Methods Fifty-four New Zealand rabbits were randomly divided into normal group, denervated group and braking group, each group was given three sampling time points of 4 weeks, 8 weeks and 12 weeks. There were 18 rabbits in each group and 6 rabbits at each time point. In the denervated group, the brachial plexus of the right upper limb was completely severed. In the braking group, the right upper limb was firmly fixed by plaster and plaster bracket. Main outcome measures: At 4, 8 and 12 weeks after modeling, the right humerus of rabbits was taken out for examination, the wet weight of the whole humerus was weighed, the mechanical properties of the humerus were measured by three-point bending test, the bone mineral density was measured by dual-energy X-ray absorptiometry, and the fine structure of bone tissue was observed by HE staining. Results BMD in denervated group and braking group was lower than that in normal group at the same time (P<0.05). The BMD of humerus in denervated group was lower than that in braking group, but the difference was not statistically significant (P>0.05). Compared with the braking group, the maximum load and bending strength of the denervated group were lower than those of the braking group at all time points after modeling, but the difference was only significant at the 12th week (P<0.05). The wet weight of humerus in the denervation and braking groups decreased with time. However, there was no significant difference in wet weight of humerus between denervated group and braking group (P>0.05). Compared with bone histomorphology of normal group, bone trabeculae became thinner in denervated group and braking group, and there were obvious fracture between trabeculae, and a large number of vacuolar structures. Conclusion Denervation and braking can cause severe osteoporosis. In terms of the degree of osteoporosis caused by both, denervation has a more serious effect on the mechanical properties of bone than braking. Lower mechanical load plays a more important role in the process of osteoporosis. |
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