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| 淫羊藿和女贞子配伍对绝经后骨质疏松症大鼠TGF-β1/Smads信号通路的实验研究 |
| Effects of the combination of Herba Epimedii and Fructus Ligustri Lucidi on TGF-β1/Smads signaling pathways in ovariectomy-induced osteoporosis rats |
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| DOI：10.3969/j.issn.1006-7108.2019.12.004 |
| 中文关键词: 淫羊藿/女贞子 绝经后骨质疏松症 转化生长因子-β1 Smads蛋白 |
| 英文关键词:Herba Epimedii/Fructus Ligustri Lucidi postmenopausal osteoporosis TGF-β1 Smads |
| 基金项目:国家自然科学基金项目( 81673993)；国家中医药管理局全国名老中医药专家传承工作室建设项目 |
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| 中文摘要: |
| 目的 研究淫羊藿和女贞子配伍对绝经后骨质疏松症（postmenopausal osteoporosis，PMOP）大鼠转化生长因子（transforming growth factor，TGF）-β1/Smads信号通路的影响。方法 将48只SPF级8月龄雌性SD大鼠，随机分为假手术组、模型组、淫羊藿组、女贞子组、淫羊藿和女贞子配伍组（配伍组）、雷洛昔芬组。对大鼠行双侧卵巢切除术造模，术后1周开始给予相应的药物治疗至术后13周结束。运用酶免法检测血清TGF-β1含量、蛋白免疫印迹法（western blot，WB）和免疫荧光法（immunofluorescence staining，IF）检测骨组织中TGF-β1、p-Smad2/3和Smad7蛋白表达，通过实时荧光定量PCR（quantitative real-time PCR，qRT-PCR）法检测骨组织中TGF-β1、Smad2、Smad3和Smad7 mRNA的表达。结果 与模型组比较，淫羊藿、女贞子配伍组能增加大鼠血清TGF-β1含量（P<0.05），上调骨组织中TGF-β1、Smad2、Smad3的mRNA表达和TGF-β1、p-Smad2/3的蛋白表达（P均< 0.01），下调骨组织中Smad7的mRNA和蛋白表达（P均< 0.01），且配伍组对上述指标的调节作用优于淫羊藿或女贞子单用。结论 淫羊藿和女贞子配伍可以调节PMOP大鼠TGF-β1/Smads信号通路中细胞因子的表达，从而调节骨代谢。 |
| 英文摘要: |
| Objective To investigate the effects of the combination of Herba Epimedii and Fructus Ligustri Lucidi on TGF-β1/Smads signaling pathways in ovariectomy (OVX)-induced osteoporosis of rats. Method PMOP rat model was duplicated by bilateral ovariectomy. Rats were randomly divided into 6 groups, including sham group, model group, Herba Epimedii group, Fructus Ligustri Lucidi group, combination group and raloxifene group. TGF-β1 in blood, the protein expression of TGF-β1, p-Smad2/3 and Smad7 in bone, and the mRNA expression of TGF-β1, Smad2, Smad3 and Smad7 in bone were detected. Results Compared with the model group, Herba Epimedii and Fructus Ligustri Lucidi in combination could significantly up-regulated the level of serum TGF-β1, the mRNA expression of TGF-β1, Smad2 and Smad3 and the protein expression of TGF-β1 and p-Smad2/3 in bone tissue, and reduced the expression of mRNA and protein of Smad7 in bone tissue (P<0.05 or P<0.01). Conclusion Herba Epimedii and Fructus Ligustri Lucidi in combination has the anti-osteoporosis effect on PMOP by regulate the expression of cytokines in TGF-β1/Smads signaling pathway. |
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