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| 长链非编码RNA GAS5在绝经后骨质疏松症肾阴虚证的表达及对骨免疫细胞因子的调控作用 |
| Expression of long-chain non-coding RNA GAS5 in postmenopausal osteoporosis with kidney Yin deficiency syndrome and its effect on bone immune cytokines |
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| DOI：10.3969/j.issn.1006-7108.2019.12.005 |
| 中文关键词: 中医中药 绝经后骨质疏松症 肾阴虚证 长链非编码RNA GAS5 细胞因子 骨免疫 |
| 英文关键词:Chinese medicine kidney Yin deficiency postmenopausal osteoporosis lncRNA GAS5 cytokine bone immunity |
| 基金项目:国家自然科学基金（81774350） |
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| 中文摘要: |
| 目的 研究长链非编码RNA GAS5 (long non-coding RNA GAS5,lncRNA GAS5)在绝经后骨质疏松症（postmenopausal osteoporosis，PMOP）肾阴虚证的表达及其对骨免疫相关细胞因子的调控作用。方法 随机选择绝经后妇女，经骨密度检测和中医辨证分型，分为肾阴虚证组（38例）、肾阳虚证组（32例）和健康对照组（35名）。通过实时荧光定量PCR（qRT-PCR）检测外周血单核细胞中lncRNA GAS5的表达。通过慢病毒介导的RNA干扰技术，抑制THP-1人单核细胞中lncRNA GAS5基因的表达，并应用细胞因子抗体芯片技术检测骨免疫相关细胞因子的表达变化。结果 与对照组比较，lncRNA GAS5在PMOP肾阴虚证组和肾阳虚证组的表达水平均明显降低（P<0.01）；与肾阳虚证组比较，肾阴虚证组lncRNA GAS5的降低趋势更明显（P<0.05）；RNA干扰抑制lncRNA GAS5表达后，与对照组比较，干扰组lncRNA GAS5的表达下调67.58%，而白细胞介素(IL-6、IL-7、IL-17)、转化生长因子β(TGF-β1)、集落刺激因子（G-CSF 、M-CSF）等23种骨代谢相关细胞因子的表达水平显著上调，重组人巨噬细胞炎症蛋白-1β（MIP-1b）、白细胞介素1 b （IL-1 b）、胞间黏附分子-1（ICAM-1）等3种因子的表达下调。结论 lncRNA GAS5在PMOP肾阴虚证中低表达；体外抑制lncRNA GAS5的表达可影响骨免疫相关细胞因子水平。 |
| 英文摘要: |
| Objective To study the expression of long non-coding RNA GAS5 (lncRNA GAS5) in postmenopausal osteoporosis (PMOP) with kidney Yin deficiency syndrome and its regulation on bone immune related cytokines. Methods Postmenopausal osteoporosis patients were randomly selected and divided to kidney Yin deficiency syndrome group (38 cases) and kidney yang deficiency syndrome group (32 cases) according to the TCM syndrome differentiation. Thirty-five healthy postmenopausal women were selected as normal control group. lncRNA GAS5 expression in peripheral blood mononuclear cells was detected using real-time fluorescence quantitative PCR (qRT-PCR). The expression of lncRNA GAS5 gene was inhibited by lentiviral-mediated RNA interference in THP-1 human monocytes. The cytokine antibody chip technology was used to detect the expression changes of bone immune-related cytokines after down-regulation of lncRNA GAS5. Results The expression level of lncRNA GAS5 was significantly lower in the PMOP kidney yin deficiency syndrome group and the kidney yang deficiency syndrome group (P<0.01) than in the control group. Compared with the kidney yang deficiency syndrome group, the kidney yin deficiency syndrome group had a lower tendency of lncRNA GAS5. Significantly (P<0.05). After inhibition of lncRNA GAS5 expression with RNA interference, lncRNA GAS5 expression was down-regulated by 67.58% in the interference group compared with that in the control group. The expression levels of interleukin (IL-6, IL-7, IL-17 ), transforming growth factor beta (TGF-β1), colony stimulating factor (M-CSF, G-CSF), and other bone metabolism-related factors were significantly up-regulated, but MIP-1b, IL-1 b, and ICAM- 1 expressions were downregulated. Conclusion lncRNA GAS5 is lowly expressed in PMOP kidney yin deficiency syndrome. Inhibition of lncRNA GAS5 expression affects bone immune-related cytokine levels in vitro. |
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