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| 降钙素基因相关肽在去势联合I/R大鼠海马和骨组织中表达的关联性研究 |
| Correlation between calcitonin gene-related peptide expression in hippocampus and bone tissue of castration combined with I/R rats |
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| DOI：10.3969/j.issn.1006-7108.2019.12.019 |
| 中文关键词: 降钙素基因相关肽 骨质疏松 脑缺血再灌注(I/R) 去势 海马 股骨头 |
| 英文关键词:CGRP osteoporosis ischemia-reperfusion hippocampus ovariectomized femoral head |
| 基金项目:福建省中青年教师教育科研项目（JAT170517） |
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| 中文摘要: |
| 目的 探讨降钙素基因相关肽（calcitonin gene-related peptide ,CGRP）在去势大鼠合并脑缺血再灌注(I/R)中的损伤海马与骨质疏松股骨头中的跨种属组织关联性现象。方法 建立去势大鼠模型后，采用线栓法建立短暂性右侧大脑中动脉闭塞海马损伤(I/R)模型，随机将大鼠分为正常组、假手术组、去势组、I/R组和去势合并I/R组。20 d后通过HE染色法观察海马和股骨头组织病理形态学的变化情况，通过ELISA测定血清、海马及股骨头组织中CGRP表达。结果 与正常组相比，去势组、I/R组和合并组骨血清 CGRP水平均明显下降，差异有统计学意义（P＜0.05）;与正常组相比，I/R组和合并组中海马CGRP水平明显上升（P＜0.05）;与正常组相比，去势组和合并组中骨组织CGRP水平明显升高（P＜0.05）;与正常组相比，I/R组、合并组海马神经元排列杂乱无序，层次混乱，形态异常，细胞数较正常组显著降低（P＜0.05）;与正常组相比，去势组成骨细胞数明显下降（P＜0.05）。结论 CGRP在去势联合I/R大鼠中的海马和骨组织表达具有正相关性，CGRP介导了神经系统参与骨组织代谢的调节、骨修复与改建，具有跨种属促进改善骨质疏松的作用。 |
| 英文摘要: |
| Objective To investigate the association of calcitonin gene-related peptide (CGRP) in cross-species tissue in injured hippocampus and osteoporotic femoral head in castrated rats with cerebral ischemia-reperfusion (I/R) phenomenon. Methods After establishing a castrated rat model, a transient right middle cerebral artery occlusion model was established by wire bolting. The rats were randomly divided into normal group, sham operation group, castration group, I/R group and castration combined with I/R group. After 15 days, HE staining was used to observe the pathological changes of hippocampus and femoral head tissues, and the expression of CGRP in serum, hippocampus and femoral head tissues was determined by ELISA. Results The levels of CGRP in the serum were significantly decreased in the castration group, the I/R group and the combined group compared with the normal group (P<0.05). The levels of CGRP in the hippocampus tissue were significantly increased in the I/R group and the combined group compared with the normal group (P<0.05). The levels of CGRP in the femoral head tissue were significantly decreased in the castration and the combined group compared with the normal group (P<0.05). Compared with the normal group, the hippocampal neurons in the I/R group and the combined group were disordered, had confusing levels and abnormal morphology, and significantly lower cell number (P< 0.05). The number of osteoblasts in the castration group decreased significantly compared with the normal group (P<0.05). Conclusion CGRP has a positive correlation between hippocampus and bone tissue expression in castration combined with I/R rats. CGRP mediates the involvement of the nervous system in bone tissue metabolism, bone repair and reconstruction, and has the effect of cross-species promotion to improve osteoporosis. |
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