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| SOX2基因对骨关节炎软骨细胞凋亡的影响研究 |
| Effect of SOX2 gene on apoptosis of chondrocytes in patients with osteoarthritis |
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| DOI：10.3969/j.issn.1006-7108.2019.12.022 |
| 中文关键词: 骨关节炎 软骨细胞 SOX2基因 凋亡 JAK2/STAT3信号通路 |
| 英文关键词:osteoarthritis chondrocytes SOX2 gene apoptosis JAK2/STAT3 signaling pathwa |
| 基金项目:甘肃省省青年科技基金计划项目（编号：1308RJYA050） |
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| 中文摘要: |
| 目的 探讨SOX2基因对人骨关节炎（OA）软骨细胞凋亡的影响及机制。方法 以正常软骨组织作为对照，通过Western blotting检测OA软骨组织SOX2蛋白表达。从人OA中分离软骨细胞，参照Lipofectamine TM2000说明将重组体pcDNA3.1-SOX2及空载体pcDNA3.1转染软骨细胞，并设置空白对照组。AG490作为JAK2/STAT3信号通路抑制剂，各组细胞处理48 h，通过流式细胞术、ROS试剂盒分别检测各组细胞凋亡率及ROS水平。Western blotting检测JAK2、p-JAK2、STAT3和p-STAT3的蛋白相对表达量。结果 人OA软骨组织SOX2表达明显低于在正常软骨组织表达（0.065±0.009 vs 0.313±0.028, P<0.05）。转染pcDNA3.1-SOX2的OA软骨细胞SOX2表达明显高于空白组（0.556±0.048 vs 0.122±0.013, P<0.05）。pcDNA3.1-SOX2可明显降低OA软骨细胞凋亡率(3.11±0.42 vs 8.54±0.68)及ROS水平(23.46±2.15 vs 52.67±4.41)，上调p-JAK2（0.142±0.013 vs 0.065±0.009）和p-STAT3表达（0.218±0.020 vs 0.126±0.015）（P<0.05），AG490（15.23±1.13 vs 8.15±0.62）可诱导OA软骨细胞凋亡，而pcDNA3.1-SOX2可减弱AG490对OA软骨细胞凋亡促进作用（P<0.05）。结论 SOX2可抑制OA软骨细胞凋亡，其机制可能与激活JAK2/STAT3信号通路有关。 |
| 英文摘要: |
| Objective To investigate the effect of SOX2 gene overexpression on chondrocyte apoptosis of human osteoarthritis (OA). Methods Normal cartilage tissue was used as control. Western blotting was used to detect the expression of SOX2 in OA cartilage tissue. Chondrocytes were isolated from human OA and recombinant pcDNA3.1-SOX2 and empty vector pcDNA3.1 were transfected into the chondrocytes according to Lipofectamine TM2000. The blank group was set up. AG490 was used as JAK2/STAT3 signal pathway inhibitor. Cells were treated for 48h. The apoptotic rate and ROS level were detected with flow cytometry and ROS kit, respectively. Western blotting was used to detect the expression of JAK2, p-JAK2, STAT3, and p-STAT3 protein. Results The expression of SOX2 in human OA cartilage tissue was significantly lower than that in normal cartilage tissue (0.065±0.009 vs 0.313±0.028, P<0.05). The expression of SOX2 in OA chondrocytes transfected with pcDNA3.1-SOX2 was significantly higher than that in blank group (0.556±0.048 vs 0.122±0.013, P<0.05). pcDNA3.1-SOX2 significantly reduced the apoptotic rate (3.11±0.42 vs 8.54±0.68) and ROS level (23.46±2.15 vs 52.67±4.41) of OA chondrocyte, but promoted the expression of p-JAK2 (0.142±0.013 vs 0.065±0.009) and p-STAT3 (0.218±0.020 vs 0.126±0.015) (P<0.05), AG490 (15.23±1.13 vs 8.15±0.62) significantly induced apoptosis of OA chondrocytes. pcDNA3.1-SOX2 weakened the apoptotic effect of AG490 on OA chondrocytes (P<0.05). Conclusion SOX2 inhibits the apoptosis of OA chondrocytes and protects OA from injury. The mechanism is related to the activation of JAK2/STAT3 signaling pathway. |
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