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| 宁夏回、汉老年女性原发性骨质疏松症的维生素D受体基因多态性研究 |
| Study on gene polymorphism of vitamin D receptor in elder female Han and Hui patients with primary osteoporosis in Ningxia |
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| DOI：10.3969/j.issn.1006-7108.2019.12.023 |
| 中文关键词: 维生素D受体基因多态性 汉族 回族 骨质疏松症 |
| 英文关键词:vitamin D receptor gene polymorphism Han nationality Hui nationality osteoporosis |
| 基金项目:宁夏回族自治区自然科学基金(NZ15184) |
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| 中文摘要: |
| 目的 探讨宁夏地区回、汉老年女性原发性骨质疏松症（primary osteoporosis，PO）维生素D受体基因（vitamin D receptor, VDR）基因多态性分布。方法 应用聚合酶链反应限制性片段长度多肽性技术（polymerase chain reaction-restrictive fragment length polymorphism, PCR-RFLP），从2015年至 2018年筛选宁夏医科大学总医院门诊及住院患者，收集119例汉族和111例回族原发性骨质疏松症妇女的血液样本并进行VDR基因型的分型检测，探讨宁夏回、汉VDR基因多态性是否存在差异。结果 111例患者的VDR受体基因型频率分布均符合Hardy-Weinberg定律，汉族和回族的女性骨质疏松症的bb 型占89.20%，68.21%。Bb基因型频率分别为8.79%和27.16%，BB基因型频率为0.00%和3.80%。宁夏回、汉女性原发性骨质疏松症中VDR基因型频率分布差异具有统计学意义（P<0.001）。结论 宁夏回、汉女性原发性骨质疏松症患者VDR基因多态性存在民族差异性，这种民族差异性与骨质疏松症的相关性有待于进一步研究。 |
| 英文摘要: |
| Objective To investigate the distribution of gene polymorphism of vitamin D receptor (VDR) of Han and Hui nationality population in Ningxia. Methods Two hundred and thirty specimens of female outpatients were collected from the clinic of General Hospital of Ningxia Medical University from 2015 to 2018. All specimens were divided into Han nationality osteoporosis group (A group, n=119) and Hui nationality osteoporosis group (B group, n=111), according to the diagnostic criteria of osteoporosis. The genotype of VDR was measured using polymerase chain reaction-restriction fragment length polymorphism (PCR-RELP). Results The distribution of nucleotide polymorphism in VDR was paralleled with the Hardy-Weinberg rule. Genotype bb in Han and Hui nationality was 89.20% and 68.21%, respectively. Bb genotype was 8.79% and 27.16%, respectively. BB genotype was 0.00% and 3.80%, respectively. Statistical analysis showed there was a significant difference of VDR gene polymorphism between Han nationality and Hui nationality (P<0.001). Conclusion There is a significant difference of VDR gene polymorphism between Han and Hui nationality. The present study may contribute to a basis for the further research in the effect of diverse ethnics on osteoporosis. |
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