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| 基于16S rRNA高通量测序技术分析I型骨质疏松大鼠肠道菌群结构变化 |
| Analysis of the gut microbiota changes in ovariectomized rats based on 16S rRNA high throughput gene sequencing |
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| DOI：10.3969/j.issn.1006.7108.2020.03.012 |
| 中文关键词: 骨质疏松症 肠道菌群 去势大鼠 16S rRNA |
| 英文关键词:osteoporosis gut microbiota ovariectomized rats 16S rRNA |
| 基金项目:上海交通大学医学院附属第九人民医院交叉学科基金项目（JYJC201809）；上海市临床医学中心（2017ZZ01023）；上海市临床重点专科。 |
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| 中文摘要: |
| 目的 探讨I型骨质疏松大鼠肠道菌群的结构变化。方法 采用去势法建立I型骨质疏松大鼠模型（n=24）：雌性SD大鼠随机分为4组（n=6），实验动物自由进食水，12 h光照周期。分别在去势前和去势后4、8、12周共4个时间点，采用直接收集法采集大鼠粪便。利用16S rRNA高通量肠菌基因测序技术，在phylum（门），class（纲），order（目），family（科），genus（属），species（种）级别分析肠道菌群的变化规律。结果 厚壁菌门与拟杆菌门是肠道菌群的主要构成肠菌，无论在去势前或去势后，二者含量之和均超过肠道菌群总量的90%。厚壁菌门含量在去势后下降并且持续到术后8周，其含量在术后12周显著增加（P<0.05）；拟杆菌门的含量变化趋势与此相反。另外，在纲、目级别的物种差异变化趋势较为一致。普雷沃氏菌属、Odoribacter 含量在术后12周明显降低（P<0.05），而螺杆菌属与Alistipes在术后12周含量显著增多（P<0.05）。在种级别的分类中，白色瘤胃球菌在术后12周显著增多（P<0.05），而单形拟杆菌含量显著降低（P<0.05）。结论 I型骨质疏松大鼠的肠道菌群在不同分类级别的结构组成上发生了显著性改变，为研究肠道菌群和骨质疏松症的关系提供理论依据。 |
| 英文摘要: |
| Objective To investigate the changes of the gut microbiota in rats with type I osteoporosis. Methods Ovariectomy was used to establish the rat model of type I osteoporosis (n=24). Female SD rats were randomly divided into 4 groups (n=6 each). The experimental animals were freely access to water and food and had a 12-hour light cycle. The rat feces were collected by direct collection method before ovariectomy and at 4, 8, and 12 weeks post ovariectomy. High throughput 16S rRNA sequencing technology was used to analyze the changes of intestinal flora at phylum, class, order, family, genus, and species level. Results Firmicutes and Bacteroides were the main components of the intestinal flora. The total content of both bacteria was more than 90% of that of intestinal flora before and after ovariectomy. The content of Firmicutes decreased after ovariectomy and lasted until 8 weeks after operation. The content of Firmicutes increased significantly at 12 weeks after operation (P<0.05). The trend of change of Bacteroides was opposite. In addition, the results of species differences at class and order levels were consistent. The contents of Prevotella and Odoribacter decreased significantly at 12 weeks after operation (P<0.05), while the contents of Helicobacter and Alistipes increased at 12 weeks after operation (P<0.05). In the classification of species level, Ruminococcus albus increased significantly 12 weeks after operation (P<0.05), while the content of Bacteroides uniformis decreased significantly (P<0.05). Conclusion The composition and structure of gut microbiota changed significantly after ovariectomy at different taxonomic levels in rats with type I osteoporosis, which might provide theoretical basis for the study of the relationship between gut microblota and osteoporosis. |
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