| Objective To study the effect of parathyroid hormone combined with calcitonin on bone-related growth factor and fracture healing in rats with osteoporotic fracture. Methods 75 female SD rats were randomly divided into: sham operation group, ovariectomized group, parathyroidism group, calcitonin group, and combination drug group, with 15 rats in each group. Firstly, an animal model of osteoporotic fracture in ovariectomized rats was constructed. Fracture healing was observes and evaluated, osteophyte BMD and BMC were determined, serum BMP-2, VEGF, TGF-β, IGF-1 concentration and osteopontin expression were assessed, and the morphology of the bone callus was observed. Results Compared with the sham operation group, the fracture healing score, BMD and BMC, serum BMP-2, VEGF, TGF-β, IGF-1 and osteopontin expression were significantly lower in the ovariectomized group (P<0.05). The above-mentioned indicators in the combination group were significantly higher than the ovariectomized group (P<0.05). In the ovariectomized group, the trabecular bone of the osteophyte was significantly reduced, the growth was sparse, the arrangement was disordered, there was a large number of mesenchymal stem cells and fibrocartilage cells, whereas osteoblasts and microvessels were rare. The combination group had a large number of trabecular bones, which grew vigorously, and were interlaced with each other. The arrangement was dense and orderly, and more mature bone cells and osteoblasts could be seen. Conclusion Parathyroid hormone combined with calcitonin mediated the expression of related bone growth factors, increased bone mineral density and mineral content of osteoporotic fractures, improved bone histomorphology and accelerated fracture healing. |