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| 固本增骨方含药血清对骨髓间充质干细胞成骨分化的影响 |
| Effect of Gubenzenggu drug-containing serum on osteogenic differentiation of rat bone marrow stromal cells |
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| DOI：10.3969/j.issn.1006.7108.2020.04.009 |
| 中文关键词: 骨质疏松 固本增骨方 含药血清 骨髓间充质干细胞 成骨分化 大鼠 动物实验 |
| 英文关键词:osteoporosis Gubenzenggu drug-containing serum bone marrow mesenchymal stem cells osteogenic differentiation rats animal experimentation |
| 基金项目:甘肃省自然科学基金计划（17JR5RA056，1506RJZA048 )；甘肃省中医药防治慢性疾病重点实验室开放基金项目 (GSMBKY2015-07)；甘肃省中医药管理局科研课题（GZK-2016-17)；肃省中药现代制药工程研究院项目（YWW-2015049) |
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| 中文摘要: |
| 目的 观察固本增骨方含药血清对大鼠骨髓间充质干细胞成骨分化的作用并探讨其可能的作用机制。方法 制备固 本增骨方含药血清，将大鼠骨髓间充质干细胞（bone marrow mesenchymal stem cells，BMSCs)分为空白血清组、5%、10%、20%、 30%浓度含药血清组及传统诱导组，分别对各组大鼠BMSCs进行成骨诱导。4周后进行茜素红染色镜下观察各组红色钙结节数量，Western blotting法分析各组BGP、Runx2的蛋白相对表达量，Real-Time PCR检测各组中BGP、Runx2 mRNA的相对表达 量。结果 各组BMSCs成骨诱导分化后的形态变化各有特点，茜素红染色后镜下观察，红色钙结节数量随含药血清浓度增加而增加，并且浓度为20%时钙结节数量明显多于其他各组（P<0.01) ；BGP、Runx2的蛋白及mRNA相对表达量在20%浓度含药血清组达最髙，且与其他各组比较差异均有统计学意义（P<0. 01)。结论 固本增骨方含药血清能通过BGP、Runx2的转录与表达促进大鼠BMSCs成骨分化，且在20%浓度为最佳。 |
| 英文摘要: |
| Objective To observe the effect of Guben Zenggu Recipe on osteogenic differentiation of rat bone marrow mesenchymal stem cells and explore its possible mechanism. Methods The serum containing Guben Zenggu Recipe was prepared. The BMSCs of rats were divided into blank serum group, 5%, 10%, 20% and 30% concentration drug-containing serum group and traditional induction group for osteoinduction. After 4 weeks ’ the number of red calcium nodules in each group was observed under alizarin red staining. The relative expression of BGP and Runx2 protein in each group was analyzed by Western blotting. The relative expression of BGP and Runx2 mRNA in each group was detected by Real-Time PCR. Results The morphological changes of BMSCs after osteogenic induction and differentiation were different in each group. After staining with alizarin red，the number of red calcium nodules increased with the concentration of drug-containing serum， and the number of calcium nodules was the highest at 20% concentration. Compared with other groups (P <0.01), the 20% concentration drug-containing serum group had the highest relative expression levels of BGP and Runx2 protein and mRNA，and the differences were statistically significant (P<0.01). Conclusion The serum containing Guben Zenggu Formula could promote the osteogenic differentiation of BMSCs in rats through the transcription and expression of BGP and Runx2 ’ and the best concentration is 20%. |
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