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| 盐酸二甲双胍抑制破骨细胞分化的研究 |
| Study on the effect of metformin hydrochloride on osteoclast differentiation |
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| DOI：10.3969/j.issn.1006-7108.2020.05.006 |
| 中文关键词: 盐酸二甲双胍 破骨细胞 分化 |
| 英文关键词:metformin hydrochloride osteoclasts differentiation |
| 基金项目:广州医科大学大学生实验室开放项目（2018年度） |
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| 中文摘要: |
| 目的 探讨盐酸二甲双胍对破骨细胞分化抑制作用的研究。方法 核因子-κB 受体活化因子配体(RANKL)诱导小鼠骨髓巨噬细胞分化成破骨细胞，在诱导过程中加入不同浓度的盐酸二甲双胍。培养7 d后固定，抗酒石酸酸性磷酸酶染色，计数破骨细胞的数量，骨吸收培养板观察骨陷窝面积，Western blot检测盐酸二甲双胍对ERK磷酸化的影响。结果 盐酸二甲双胍能抑制RANKL诱导的破骨细胞的形成，并且能减少骨陷窝面积，以及抑制ERK磷酸化。结论 盐酸二甲双胍可抑制破骨细胞分化及功能，其机制可能与抑制ERK磷酸化有关。 |
| 英文摘要: |
| Objective To investigate the inhibitory effect of metformin hydrochloride on the differentiation of osteoclasts. Methods Mouse bone marrow macrophages were induced with receptor activator of nuclear factor κB ligand (RANKL) and then cultured with different concentrations of metformin hydrochloride for 7 d. Osteoclast number was counted with TRAP staining. The function of osteoclasts was examined with hydroxyapatite-coated plates. The effect of metformin hydrochloride on phosphorylation of ERK was evaluated with Western blotting. Results Metformin hydrochloride inhibited the differentiation of osteoclasts, decreased the bone resorption area, and suppressed phosphorylation of ERK. Conclusion Metformin hydrochloride inhibits osteoclastogenesis and osteoclast function probably through suppressing phosphorylation of ERK. |
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