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| 血清25-(OH)D水平及VDR基因多态性与类风湿性关节炎骨侵蚀的关系探讨 |
| Relationship among serum 25- (OH) D level, VDR gene polymorphism, and bone erosion in patients with rheumatoid arthritis |
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| DOI：10.3969/j.issn.1006-7108.2020.07.011 |
| 中文关键词: 25-羟维生素D 维生素D受体 基因多态性 类风湿性关节炎 骨侵蚀 |
| 英文关键词:25-hydroxyvitamin D vitamin D receptor gene polymorphism rheumatoid arthritis bone erosion |
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| 中文摘要: |
| 目的 探讨血清25-羟维生素D［25-(OH)D］及维生素D受体(vitamin D receptor，VDR)基因多态性与类风湿性关节炎骨侵蚀的关系。方法 将94例类风湿性关节炎患者设为研究组，另将85名健康体检者设为健康组。2组均检测血清25-(OH)D水平，且提取基因组DNA，采用MassARRAY分子量阵列技术平台检测VDR基因多态性，分析其与类风湿性关节炎骨侵蚀度之间的关系。结果 研究组血清25-(OH)D水平低于健康组（P＜0.05），且骨侵蚀患者血清25-(OH)D水平低于无骨侵蚀者（P＜0.05）；研究组FokI位点基因型FF、Ff及等位基因F和ApaI位点基因型AA、Aa及等位基因A频率均高于健康组（P＜0.05）；研究组ff、aa出现频率均低于健康组（P＜0.05）；血清25-(OH)D＜30 ng/mL、FokI位点基因型FF及Ff、ApaI位点基因型AA及Aa均是类风湿性关节炎发病的危险因素（P＜0.05），血清25-(OH)D＜20 ng/mL是类风湿性关节炎骨侵蚀的危险因素（P＜0.05）。结论 血清25-(OH)D水平缺乏可增加类风湿性关节炎骨侵蚀发生风险，VDR基因FokI位点等位基因F及ApaI位点等位基因A可增加类风湿性关节炎发病风险，但与骨侵蚀无关。 |
| 英文摘要: |
| Objective To explore the relationship among serum 25-hydroxyvitamin D [25-(OH)D], vitamin D receptor (VDR) gene polymorphism, and bone erosion in patients with rheumatoid arthritis. Methods Ninety-four patients with rheumatoid arthritis were divided into study group and 85 healthy persons were divided into health group. The serum 25- (OH)D levels were detected in the 2 groups. The genomic DNA was extracted and the VDR gene was detected using MassARRAY molecular weight array technology platform. The relationship between serum 25-(OH)D level and VDR gene polymorphism and bone erosion in rheumatoid arthritis was analyzed. Results The serum 25-(OH)D level in the study group was significantly lower than that in the healthy group (P<0.05), and it was lower in the patients with bone erosion than in the patients without bone erosion (P<0.05). The frequencies of genotype FF, Ff, and allele F of FokI locus and genotype AA, and allele A of ApaI locus in the study group were significantly higher than those in the healthy group (P<0.05). The frequencies of genotype ff and aa in the study group were significantly lower than those in the healthy group (P<0.05). Serum 25-(OH) D less than 30 ng/mL, FokI genotype FF and Ff, and ApaI genotype AA and Aa were risk factors for rheumatoid arthritis (P < 0.05). Serum 25-(OH)D less than 20 ng/mL was a risk factor for bone erosion in rheumatoid arthritis (P<0.05). Conclusion Lack of serum 25-(OH)D increases the risk of bone erosion in patients with rheumatoid arthritis. The allele F of FokI and the allele of ApaI of VDR gene increase the risk of rheumatoid arthritis, but VDR gene is not associated with bone erosion. |
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