| Objective To explore the potential mechanism of Astragalus treatment of osteoporosis based on network pharmacology. Methods The potential chemical components and target prediction of Astragalus were obtained in TCMSP database. GeneCards and OMIM database were used to predict and screen osteoporosis-related genes, and R software was used to match drug targets with disease targets. The drug-component-target-disease visualization network was established by Cytoscape. The Protein Interaction Network was constructed by using the String database online platform. The DAVID database was used for biological process and pathway analysis. Results A total of 20 major ingredients of Astragalus were obtained after screening, and 218 targets were predicted. 22 targets related to osteoporosis, mainly affecting transcription factor activity, ligand activity, cytokine activity, steroid hormone receptor activity ,etc and involved in rheumatoid arthritis pathway, osteoclast differentiation pathway, IL-17 factor pathway, AGE-RAGE signaling pathway, estrogen pathway, etc.Conclusion The results of this study confirmed that Astragalus has multiple components, multiple targets, and multiple processes. It is preliminarily stated that it is not only directly involved in bone metabolism, but also indirectly through the digestive system, circulatory system, endocrine system and other systems regulates environmental of bone metabolism in the treatment of osteoporosis;The enrichment of the Toll-like receptor signaling pathway, inflammatory bowel disease, PI3K-Akt signaling pathway and other related pathways of the digestive system and spleen may be important evidence for the treatment of osteoporosis from the spleen. |