| Objective To investigate the effect of artificial tiger bone powder on Sirt1/Runx2 signaling pathway in bone tissue of ovariectomized rats.Methods Forty adult female SD rats were selected to establish ovariectomized rat model. After ovariectomy for 2 weeks, they were divided into artificial tiger bone powder group and ovariectomized group. The artificial tiger bone powder group was given 0.24 g/(kg·d) by gavage, and the ovariectomized group was given equivalent dose of saline by gavage. In addition, 20 adult female SD rats were taken out, only the adipose tissue around ovary was removed, and bilateral ovaries were not removed. After 2 weeks of resection, the adipose tissue around ovary was given the equivalent dose of saline by intragastric administration. After 12 weeks of continuous administration, femoral density, bone metabolism, bone biomechanics, femoral histology and Western Blot were measured.Results Compared with the sham-operated group, the femoral density, maximum load, fracture load, maximum stress, elastic modulus and the expressions of Sirt1, Runx2, Foxo1 and Foxo3a protein in femoral tissue of ovariectomized group decreased, while the concentrations of osteocalcin, tartrate-resistant acid phosphatase, serum calcium, phosphorus and alkaline phosphatase increased, and the femoral tissue presented osteoporosis. Physical changes; Compared with ovariectomized group, femoral density, maximum load, fracture load, maximum stress, elastic modulus and expressions of Sirt1, Runx2, Foxo1 and Foxo3a in femoral tissue of artificial tiger bone powder group increased, while osteocalcin, tartrate-resistant acid phosphatase, serum calcium, phosphorus and alkaline phosphatase decreased, and osteoporosis decreased. Pathological changes were significantly improved.Conclusion Artificial tiger bone powder may play an anti-osteoporosis role by regulating Sirt1/Runx2 signaling pathway. |