| Objective To study the effect of miR-92a-3p targeting EZH2 on the synthesis of matrix degrading enzymes in chondrocytes induced by IL-1β. Methods Human chondrocytes were divided into normal, IL-1β (treatment of IL-1β), miR-NC+IL-1β (treatment of IL-1β after mimics control transfection), and miR-92a-3p+IL-1β (treatment of IL-1βafter miR-92a-3p mimics transfection) group. The expression of miR-92a-3p was determined using RT-PCR. Western blotting was used to detect the expression of MMP-13, MMP-1, and col Ⅱ. It was predicted with a software that EZH2 might be the target gene of miR-92a-3p. The target relationship was identified by luciferase report system. pCDNA3.1-EZH2 and miR-92a-3p mimics were co-transfected into chondrocytes. The protein expressions of MMP-13, MMP-1, and col Ⅱ were detected. Results Compared with those in normal group, the expression of miR-92a-3p decreased, the expressions of MMP-13 and MMP-1 increased, and the expression of col Ⅱ decreased in IL- β group (P< 0.05). Compared with those in miR-NC+ IL-1β group, the expression of miR-92a-3p increased, the expressions of MMP-13 and MMP-1 reduced, and the expression of col Ⅱ increased in miR-92a-3p + IL-1β group (P<0.05). miR-92a-3p negatively target-regulated EZH2 expression. pCDNA3.1-EZH2 reversed the effect of miR-92a-3p mimics on the expressions of MMP-13, MMP-1, and col Ⅱ in chondrocytes with the condition of IL-1β. Conclusion miR-92a-3p targeted down-regulation of EZH2 inhibits IL-1β- induced matrix degrading enzyme synthesis in chondrocytes. |