中国骨质疏松杂志

绝经后血清BMP-7、OCN水平与骨质疏松的相关性研究

Study on the correlation between the levels of serum BMP-7 and OCN and osteoporosis in postmenopausal women

DOI：10.3969/j.issn.1006-7108.2020.09.012

英文关键词:bone morphogenetic protein-7 osteocalcin menopause osteoporosis correlation

基金项目:国家自然科学基金项目（骨细胞程序性坏死通过上调RANKL促发绝经后骨质疏松发生的机制研究）(81760260)

作者	单位
余丽金1 温广宇1* 崔红旺2	1. 海南现代妇女儿童医院，海南海口57000 2. 海南医学院第一附属医院，海南海口 57000

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中文摘要:

目的探究骨形态发生蛋白-7（BMP-7）、骨钙素(OCN)在绝经后骨质疏松（OP）患者血清中的水平与骨代谢指标骨特异性碱性磷酸酶（BAP）、I型胶原交联氨基末端肽（NTX）及骨密度（BMD）的相关性。方法选取2017年5月至2019年3月收治的93例绝经女性OP患者作为OP组；并以同期来我院进行健康体检的86例绝经女性作为对照组进行研究；以实时荧光定量PCR（qRT-PCR）法检测两组受试者血清BMP-7、OCN表达水平；以酶联免疫吸附法（ELISA）检测两组受试者血清BAP、NTX水平，计算BAP/NTX比值；利用双能X线骨密度仪测定两组受试者骨密度；分析OP患者血清BMP-7、OCN表达水平与骨代谢相关指标、BMD的相关性及BMP-7表达水平与OCN的关系；Logistic回归分析OP发生的影响因素。结果 OP组患者雌二醇水平、血清BMP-7、OCN、BAP/NTX、BMD水平均明显低于对照组（t=11.962、9.969、12.823、4.052、18.215，P均<0.05）；OP组患者血清BAP、NTX水平均明显高于对照组（t=4.014、9.092，P均<0.05）；OP患者血清BMP-7、OCN表达水平与BAP、NTX均呈负相关（P均<0.05），与BAP/NTX、BMD均呈正相关（P均<0.05），BMP-7表达水平与OCN呈正相关（P<0.05）；雌二醇、BMD是影响OP发生的保护险因素（95 %CI=0.536~0.978、0.335~0.916，P均＜0.05），BAP、NTX是影响OP发生的危险因素（95 %CI=1.336~1.859、1.427~2.357，P均＜0.05）。结论绝经女性血清BMP-7、OCN表达水平下调，BMP-7、OCN与骨代谢指标及BMD密切相关，两者可能与BAP、NTX、BMD相互影响，从而在OP发展进程中发挥作用。

英文摘要:

Objective To investigate the correlation between the levels of serum bone morphogenetic protein-7 (BMP-7), osteocalcin (OCN) in postmenopausal osteoporosis (OP) patients and bone metabolism indexes of bone specific alkaline phosphatase (BAP), type I collagen cross-linked peptide (NTX) and bone mineral density (BMD). Methods Ninety-three postmenopausal women with OP treated in the hospital from May 2017 to March 2019 were selected as OP group, and 86 postmenopausal women who had health examination in the same period were selected as the control group. The general data of the two groups were compared. The serum levels of BMP-7 and OCN in the two groups were measured using real-time fluorescent quantitative PCR (qRT-PCR). The serum levels of BAP and NTX were detected using enzyme-linked immunosorbent assay (ELISA). The ratio of BAP/NTX was calculated. Bone mineral density was measured using dual energy X-ray absorptiometry. The relationship between the expression levels of BMP-7, OCN and bone metabolism related indexes, BMD, and the relationship between the expression levels of BMP-7 and OCN in OP patients were analyzed. Logistic regression was used to analyze the influencing factors of OP. Results The serum levels of estradiol, BMP-7, OCN, and BAP/NTX, and BMD in the OP group were significantly lower than those in the control group (t = 11.962, 9.969, 12.823, 4.052, 18.215, respectively, all P < 0.05). The serum levels of BAP and NTX in the OP group were significantly higher than those in control group (t = 4.014, 9.092, respectively, both P < 0.05). The expressions of BMP-7 and OCN were negatively correlated with BAP and NTX in OP patients ( all P < 0.05), and positively correlated with BAP / NTX and BMD (P < 0.05). The expression of BMP-7 was positively correlated with OCN (P < 0.05). Estradiol, BMP-7, OCN, and BMD were the protective factors of OP (95% CI = 0.536-0.978, 0.374-0.825, 0.438-0.913, 0.335-0.916, respectively, P< 0.05). BAP and NTX were the risk factors of OP (95% CI = 1.336-1.859, 1.427-2.357, respectively, P< 0.05). Conclusion The expressions of BMP-7 and OCN in postmenopausal women are down-regulated. BMP-7 and OCN are closely related to bone metabolism index and BMD, and they may interact with BAP, NTX, and BMD, and play a role in the development of OP.

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