Objective To observe the effect of bisphosphonate combined with hypoglycemic agents on the expression of BMP-2, TGF-β1 and IGF-1 in type 2 diabetic with osteoporosis rats, and to explore its mechanism of prevention and treatment of DOP. Methods 60 female SD rats were randomly divided into control group, model group, bisphosphonate group, hypoglycemic agent group, combined group, An animal model of type 2 diabetes mellitus complicated with osteoporosis was established and given different drug interventions. Glucose metabolism, bone mineral density and bone biomechanics were detected respectively, RT-PCR and immunohistochemistry were used to detect the expression of IGF-1, IRS-1 and IRS-2 mRNA and protein in bone tissue. Results The serum levels of FPG, 2hBPG and HbAlc in the bisphosphonate group, hypoglycemic group and combination group were significantly lower than the model group (P<0.05), The serum levels of FINS in the bisphosphonate group, hypoglycemic group, and combination group were significantly higher than the model group (P<0.05). The BMC and bone biomechanical index in the bisphosphonate group, hypoglycemic group, combined group were significantly higher than the model group (P<0.05). The mRNA and protein expression profiles of the bisphosphonate group, hypoglycemic group and combination group were significantly higher than the model group (P<0.05). Conclusion Bisphosphonate combined with hypoglycemic agents may regulate the expression of BMP-2, TGF-β1 and IGF-1 in type 2 diabetes mellitus with osteoporosis rats, improve glucose metabolism, increase bone density and improve bone biomechanical properties, and play a role in prevention and treatment of DOP effect. |