| Objective To explore the effects of salidroside and calcium carbonate on bone mass, bone metabolism and oxidative stress in aged female rats. Methods Forty female Sprague-Dawley (SD) rats aged 24 months were randomly divided into model group (group OP), melatonin group (group M), calcium carbonate group (group Ca) and melatonin combined with calcium carbonate group (group M+Ca), while 10 rats aged 3 months were set as the control group (group NC). The changes of bone density and bone mineral level of lumbar vertebra and bilateral femur in rats of each group were observed. The levels of serum bone metabolism indexes, superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) in rats of each group were determined. Results Compared with the OP group, the lumbar vertebrae bone density, bilateral femoral bone density, and bone mineral levels in the H, Ca, and H+Ca groups were significantly increased. After 12 weeks of treatment, serum type I collagen C in the H, Ca, and H+Ca groups The levels of telomere (CTX-1), osteocalcin, alkaline phosphatase (ALP), type I procollagen N-terminal peptide (PINP), and tartrate-resistant acid phosphatase 5b (TRACP-5b) were significantly reduced. (P <0.05); after 12 weeks of treatment, the SOD and GSH-Px activities in the H, Ca, and H+Ca groups increased significantly, and the MDA content decreased significantly; the differences were statistically significant (P<0.05 or P<0.01) Among them, the above indexes of H+Ca group had the most significant changes; the differences were statistically significant (P<0.05). Conclusions Salidroside combined with calcium carbonate can significantly improve the antioxidant capacity of aged rats, increase bone density, reduce bone metabolism, and reduce bone mineral loss, thereby preventing and treating osteoporosis. |