| Objective To explore the active components of angelica pubescens radix - loranthaceae in the treatment of osteoporosis using network pharmacology, and to explore the biological basis of angelica pubescens radix - loranthaceae in the treatment of osteoporosis through screening analysis. Methods The active components and targets of angelica pubescens radix - loranthaceae were screened from TCMSP and BATMAN database. The prediction targets of osteoporosis were retrieved from GeneCards database. The drugs and disease targets were mapped. The core components and effective targets were sorted out and screened by using the network visualization software Cytoscape 3.6.0. Then the effective targets were analyzed through OmicShare cloud platform GO enrichment analysis. KEGG pathway enrichment analysis was performed using David 6.8 database. Results According to the screening conditions, 3 core components of angelica pubescens radix - loranthaceae were obtained, including quercetin, osthol, and psoralen. There were 24 core targets, including PTGS2, PTGS1, ESR1, ADRB2, etc. There were 2509 Go biological processes, including response to oxygen-containing compound, response to organic substance, and cellular response to chemical stimulus, etc. There were 193 molecular functions, including enzyme binding, identical protein binding, signaling receptor binding, etc. There were 113 cell components, including membrane raft, membrane microdomain, membrane region, etc. Twelve KEGG signaling pathways were obtained. Tumor necrosis factor signaling pathway, hypoxia inducible factor-1 signaling pathway, and PI3K Akt signaling pathway were the key pathways for the treatment of osteoporosis. Conclusion The anti-inflammatory and anti-oxidation effects multi-component, multi-target for the treatment of osteoporosis with angelica pubescens radix - loranthaceae is through their multi-channel compatibility. This provides the scientific basis for the mechanism of action of traditional Chinese medicine compound in the treatment of diseases. |