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| 绝经后骨质疏松B细胞、T细胞亚群、免疫调控因子与骨密度相关性研究 |
| Study on the correlation among B cells, T cell subsets, immune regulatory factors and BMD in postmenopausal osteoporosis |
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| DOI：10.3969/j.issn.1006-7108.2020.12.003 |
| 中文关键词: 骨质疏松 调节性T细胞 B细胞 肿瘤坏死因子-α 转化生长因子-β 白细胞介素17 |
| 英文关键词:osteoporosis regulatory T cell B cells TNF-α TGF-β IL-17 |
| 基金项目:吉林省卫生技术创新项目（2016J039） |
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| 中文摘要: |
| 目的 研究绝经后骨质疏松（Ⅰ型骨质疏松）T细胞亚群（调节性T细胞CD3+/CD4+/CD25+）、B细胞（CD3-/CD19+）、免疫调控因子（TNF-α、TGF-β、IL-17）与骨密度相关性。阐明B细胞、T细胞亚群、免疫调控因子与骨质疏松发生、发展的关系，为骨质疏松诊断、治疗提供分子生物学依据。方法 采用双能X线骨密度仪检测受试者腰椎正位（L1~4）骨密度，采用流式细胞检测技术对B细胞、T细胞亚群进行分析，采用酶联免疫分析检测免疫调控因子。结果 绝经后骨质疏松组调节性T淋巴细胞亚群占CD4+T淋巴细胞的比例低于非骨质疏松组（P<0.05），绝经后骨质疏松组外周血B淋巴细胞（CD3-/CD19+）的百分比低于非骨质疏松组，但差异无统计学意义（P＞0.05）。绝经后骨质疏松症患者血清免疫调控因子（TNF-α、TGF-β、IL-17）水平与非骨质疏松女性差异明显（P<0.05）。结论 T淋巴细胞、B 淋巴细胞与免疫调控因子协同作用，通过影响破骨细胞、成骨细胞分化、增殖，调节骨重建。骨质疏松与机体免疫系统密切关联。 |
| 英文摘要: |
| Objective To study the correlation among postmenopausal osteoporosis (type I osteoporosis) T cell subsets (regulatory T cells CD3+/CD4+/CD25+), B cells (CD3-/CD19+), immune regulatory factors (TNF-α, TGF) -β, IL-17) and bone mineral density. Clarify the relationship among B cells, T cell subsets, immune regulatory factors and the occurrence and development of osteoporosis, and provide molecular biological evidence for the diagnosis and treatment of osteoporosis. Methods The dual-energy X-ray bone densitometer was used to detect the Lumbar vertebrae (L1-4) BMD, the flow cytometry technique was used to analyze the B cell and T cell subsets, and the enzyme-linked immunoassay was used to detect the immune regulatory factors. Results The ratio of regulatory T lymphocyte subsets to CD4+ T lymphocytes in the postmenopausal osteoporosis group was lower than that of the non-osteoporosis group (P<0.05). The peripheral blood B lymphocytes (CD3- /CD19+) percentage was lower than that of women in the non-osteoporotic group, but the difference was not statistically significant (P>0.05). The levels of serum immune regulatory factors (TNF-α, TGF-β, IL-17) in postmenopausal osteoporosis patients were significantly different from those in non-osteoporotic women (P<0.05). Conclusion T lymphocytes, B lymphocytes and immune regulatory factors work together to regulate bone remodeling by influencing the differentiation and proliferation of osteoclasts and osteoblasts. Osteoporosis is closely related to the body's immune system. |
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