| Objective To explore the mechanism of tortoise shell in the treatment of osteoporosis (OP) using network pharmacology. Methods The tortoise shell active compounds were obtained from the database of BATMAN-TCM and literatures, and then the disease related targets were obtained from the database of TTD, Drugbank, DisGeNET, and OMIM, and the tortoise shell-OP targets were obtained from the intersection of the two.GO and KEGG pathway enrichment analysis was carried out on the target gene of tortoise shell treatment osteoporosis by online analysis tool Metascape.Results 40 compounds (amino acids, fatty acids, esters, sterols, others)of tortoise shell were obtained,and 15 targets (UGT2B17, PTGS2, slc22a6, slc22a10, ptger2, SRC, COMT, TNF, ESR1, CYP19A1, ESR2, AR, PGR, ITGB3, FPDS) were screened out.The analysis of KEGG enrichment of the key target of tortoiseshell active compounds showed that it was mainly related to the signal pathways such as the neuroactive ligand-receptor interaction, aminoacyl-tRNA biosynthesis, glutamatergic synapse.The analysis of GO enrichment of tortoise shell anti osteoporosis target gene showed that it was mainly related to the response to steroid hormone, regulation of epithelial cell proliferation, and regulation of lipid location, etc.,and the analysis of KEGG pathway enrichment showed that these genes were mainly related to the steroid hormone biosynthesis, thyroid hormone signaling pathway, pathways in cancer.Conclusion The mechanism of action of tortoise shell in the treatment of OP is multi-target and multi-channel, which not only affects the related pathways of bone metabolism, but also affects many metabolic pathways in vivo. |