| Objective To investigate the effect of sesamin on osteoblast differentiation in rat bone marrow stromal cells in vitro. Methods Bone marrow mesenchymal stem cells (BMSCs) were extracted from rat femurs and induced to differentiate into osteoblasts. The Wnt/b-catenin signal pathway was silenced by FH535 (1 μ mol/L). The silent group was established, and the non-silent group was established at the same time. Both groups were intervened with sesamin (0, 1 or 10 μ mol/L) at the same time. Cell proliferation/toxicity and alkaline phosphatase (ALP), osteoblast related transcription factor antibodies (OSX), SRY-box 9 (SOX9), Runt related transcription factor 2 (RUNX2, osteocalcin (OCN), b-catenin, low density lipoprotein receptor related protein 5 (LRP5), and glycogen synthase kinase 3b (GSk-3b) were detected at the same time. Results Sesamin had no significant effect on the proliferation of BMSCs and had no cytotoxicity. Sesamin at higher concentration promoted the activity of Wnt/b-catenin signaling pathway and increased the expression of ALP, OSX, SOX9, RUNX2, and OCN. After silencing Wnt/b-catenin, the expression of RUNX2 and OCN decreased. Conclusion Sesamin has a tendency to promote rat bone marrow mesenchymal stem cells to differentiate into osteoblasts by acticating Wnt/b-catenin signal pathway, and has a certain effect on the remodeling of bone structure in rats. Sesamin has therapeutic and preventive effects on osteoporosis. |