中国骨质疏松杂志

姜黄素联合有氧运动对骨质疏松大鼠骨密度、氧化应激能力及骨组织NF-κB的影响

Effect of curcumin combined with aerobic exercise on bone mineral density, oxidative stress, and NF - κ B in bone tissue of osteoporotic rats

DOI：10.3969/j.issn.1006-7108.2021.01.011

中文关键词: 姜黄素有氧运动骨质疏松 NF-κB 大鼠

英文关键词:curcumin aerobic exercise osteoporosis NF-κB rat

基金项目:新疆维吾尔族自治区自然科学基金（2018D01C014）

作者	单位
杨青坡1 穆合塔尔·买买提热夏提1 王法正1* 高广文2 付岳松1	1.新疆维吾尔自治区喀什地区第一人民医院运动医学科，新疆喀什 844000 2.新疆维吾尔自治区哈密市中心医院骨科，新疆哈密 839000

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中文摘要:

目的探讨姜黄素联合有氧运动对骨质疏松(osteoporosis，OP)大鼠骨密度、氧化应激能力及骨组织NF-κB的影响。方法将40只SPF雌性健康大鼠通过切除双侧卵巢诱导建立OP模型，平均分成4组，Dd组（OP模型+姜黄素）、Lh组（OP模型+姜黄素+有氧运动）、OP组（OP模型）、Zc组（假手术组），通过多种方法检测不同指标水平。结果与Zc组比对，Dd组、Lh组、OP组腿股骨骨密度均下降，与OP组比对，Dd组腿股骨骨密度提升，与Dd组比对，Lh组骨密度增长（P＜0.05）。Zc组骨小梁体积百分比[（29.15±5.45）%]显著高于OP组[（10.11±4.09）%]（P＜0.05），经姜黄素干预Dd组后升高至（24.08±5.03）%，联合运动干预后Lh组为（29.55±6.33）%（P＜0.05）。GSH-Px、SOD水平从高到低依次为Zc组、Lh组、Dd组、OP组，MDA水平从高到低依次为OP组、Dd组、Lh组、Zc组（P＜0.05）。Zc组骨小梁结构良好未有明显病理改变，OP组骨小梁病理改变较重，骨小梁粗细不均，排列不规则，且有多处骨髓区未见骨小梁结构，Dd组较OP组有所好转，部分骨髓区无骨小梁结构，排列状态好于OP组，Lh组部分骨小梁结构恢复正常，骨髓区均有骨小梁结构出现，仅有少数排列不均。与Zc组比对，OP组大鼠NF-κB水平、蛋白表达上升明显，与OP组比对，Dd组、Lh组大鼠水平、蛋白表达均有所下降，且Lh组水平、蛋白表达低于Dd组（P＜0.05）。结论姜黄素联合有氧运动能明显改善OP大鼠骨密度、氧化应激能力，降低骨组织中NF-κB表达。

英文摘要:

Objective To explore the effect of curcumin combined with aerobic exercise on bone density, oxidative stress ability, and NF-κB in bone tissue of osteoporotic rats. Methods Forty SPF female healthy rats were induced to establish an OP model by removing bilateral ovaries. They were divided into 4 groups equally, Dd group (OP model + curcumin), Lh group (OP model + curcumin + aerobic exercise), OP Group (OP model), and Zc group (sham operation group). The levels of different index were detected with different methods. Results Compared with the Zc group, bone mineral density (BMD) of the femur in the Dd, Lh, and OP groups decreased. Compared with the OP group, BMD of the femur increased in the Dd group. Compared with the Dd group, BMD in the Lh group increased (P<0.05). The percentage of trabecular bone volume in the Zc group (29.15 ± 5.45)% was significantly higher than that in the OP group (10.11 ± 4.09) % (P <0.05). After curcumin intervention, it increased to (24.08 ± 5.03)% in Dd group, and it was (29.55 ± 6.33)% in Lh group (P<0.05). The levels of GSH-Px and SOD from high to low were in Zc group, Lh group, Dd group, and OP group. The levels of MDA from high to low were in OP group, Dd group, Lh group, and Zc group (P<0.05). The trabecular structure of the Zc group was good and there was no obvious pathological changes. The pathological changes in the trabecular bone in the OP group were serious. The trabecular bone was uneven in thickness and arranged irregularly. There were multiple bone marrow areas with no trabecular structure in OP group. In Dd group, some bone marrow areas had no trabecular structure, and the arrangement state was better than that in OP group. In Lh group, part of the bone trabecular structure returned to normal, and there was bone trabecular structure in the bone marrow area, with only a few uneven arrangement. Compared with that in Zc group, the NF-κB level and protein expression in OP group increased significantly. Compared with those in OP group, the NF-κB level and protein expression in Dd group and Lh group decreased, and they were lower in Lh group than in Dd group (P<0.05). Conclusion Curcumin combined with aerobic exercise significantly improves BMD and oxidative stress in OP rats, and reduces the expression of NF-κB in the bone tissue.

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