中国骨质疏松杂志

绝经后骨质疏松生物标志物的筛选及免疫细胞浸润分析

Identification of key biomarkers and analysis of immune infiltration of cells in postmenopausal osteoporosis

DOI：10.3969/j.issn.1006-7108.2021.03.013

英文关键词:postmenopausal osteoporosis infiltration of immune cells bioinformatics Gene Expression Comprehensive Database

基金项目:科技部国家重点研发计划(122300411149)

作者	单位
肖贺方 1 连笑宇1 车凌仪 2 强硕1 郑稼 1*	1.郑州大学人民医院河南省人民医院骨科，河南郑州 450003 2.郑州大学第一附属医院妇产科，河南郑州450003

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中文摘要:

目的绝经后骨质疏松症（PMOP）是绝经后妇女的常见疾病，但目前尚无有效的治疗方法。本研究旨在识别绝经后骨质疏松症形成过程中基因表达的重要变化，并提供相应的临床参考。方法从GEO下载的数据集GSE56116、京都基因和基因组百科全书（Kyoto Encyclopedia of Genes and Genomes KEGG）扩增途径、基因本体学（GO）、蛋白质-蛋白质相互作用（PPI）网络，筛选其差异表达基因（DEG），并进一步分析得到了六个关键基因，分别是FOS、SYK、HCK、SELL、CCR1、NLRP3，然后我们分析了免疫细胞浸润。结果发现在骨关节炎和正常对照之间差异很大，与正常组织相比，PMOP组织含有较低比例的活性CD4T记忆细胞（P<0.05），最后从GSE7429数据集验证了关键基因NLRP3的表达水平。结论有助于我们进一步了解PMOP形成的分子机制，并为疾病的治疗提供新的思路。

英文摘要:

Objective Postmenopausal osteoporosis (PMOP) is a usual clinical disease in postmenopausal women, but there is no effective therapy at present. This research designed to recognize the important changes in gene expression, and to provide clinical reference. Methods We downloaded gene expression dataset GSE56116 from GEO, and dissected of differentially expressed genes (DEG), Kyoto Gene and Genomic Encyclopedia (KEGG) expansion pathway, Gene Ontology (GO), and protein-protein interaction (PPI) cyber. In total, six hub genes, including FOS, SYK, HCK, SELL, CCR1, and NLRP3, were elected. Results The immune infiltration profiles varied significantly between osteoarthritis and normal controls. Compared with normal tissues, PMOP tissues contained a lower proportion of activated T cells CD4 memory (P <0.05). Finally, the expression levels of NLRP3 hub genes were confirmed with GSE7429. Conclusion In summary, this research helps us to realize the molecular mechanism of PMOP generation and offers a new direction for the treatment of PMOP.

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