| Objective To observe the expression profile of long-chain non-coding RNA (lncRNA) and its related target genes and signal pathways during the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) induced by psoralen. Methods The second generation of BMSCs were divided into three groups: blank group (l-DMEM containing 10 % FBS), osteogenic induction group (L-DMEM containing 10 % FBS and osteogenic inducers), and psoralen group (L-DMEM containing 10 μmol/L psoralen and 10% FBS). After 21-day intervention, the difference of 2-fold variation of lncRNA in the process of osteogenic differentiation was screened using Agilent lncRNA chip. Real-time fluorescent quantitative PCR was used to verify the results of the chip. The lncRNA, which was highly differentially expressed in screening results was analyzed with Go and KEGG. Results There were 446 differentially expressed lncRNAs which expressed more than 2 times continuously after 21 days of osteogenic differentiation. In the process of osteogenic differentiation of BMSCs, 5 up-regulated lncRNAs and 4 down-regulated lncRNAs were screened. Among those, XR009483 and XR007366 were most significantly up-regulated and down-regulated, respectively, and the results were confirmed with RT-PCR. The higher concentrations of GO were related to bone and cartilage development, stem cell differentiation, collagen synthesis, ossification, and calcification. Fifteen biological pathways was found in KEGG pathway analysis including TGF-beta, Wnt, NF-Kappa B, and calcium pathways. Conclusion The lncRNA expression profile of BMSCs changes significantly during osteogenic differentiation induced by psoralen, suggesting that the differential expression of lncRNA may be closely related to the osteogenic differentiation by BMSCs. |