| Objective To explore the potential molecular mechanism of Dangguibuxue decoction (DGBXD) in the treatment of Osteoporosis（OP）based on the systemic network pharmacology. Methods The main chemical components, corresponding targets and target genes of DGBXD were screened by the Traditional Chinese Medicine System Pharmacology Technology PlatformTCMSP, and the known target genes related to OP were obtained by using GeneCards database. The target genes of DGBXD obtained were mapped to the OP disease targets to get the relevant predicted targets of DGBXD acting on OP. Cytoscape3.5.1 software was used to construct the effective active compound-disease targets network diagram, while used the STRING database to draw PPI network and used the R 3.6.3 software for key proteins analysis, target genes for GO function analysis and KEGG pathway enrichment analysis. Results The results indicated 17 active chemical constituents, 7 key compounds, 130 intersectional targets from drugs mapping to the OP disease as well as screening out 30 key targets; GO functional analysis revealed that the potential genes biological functions involved in the treatment of OP by DGBXD were mainly nuclear receptor activity, transcription factor activity, direct ligand, regulated sequence-specific DNA binding, steroid hormone receptor activity, cytokine receptor binding, cytokine activity, etc; The results of KEGG pathways enrichment analysis mechanism showed that the pathways were closely related to AGE-RAGE signaling pathways in diabetic complications, IL-17 signaling pathways, Hepatitis B, Prostate cancer, TNF signaling pathways, etc. Conclusion According to this study, DGBXD can treat on the OP through multi-component, multi-target and multi-pathway, which may be related to its anti-oxidation and estrogen-like effect, and it can further provide a theoretical basis for the next experimental verification. |