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| 基于网络药理学探讨柚皮苷治疗骨质疏松症的潜在机制 |
| To investigate the underlying mechanism of Naringin in the treatment of osteoporosis on the basis of network pharmacology |
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| DOI：10.3969/j.issn.1006-7108.2021.04.012 |
| 中文关键词: 骨质疏松症 柚皮苷 网络药理学 信号通路 |
| 英文关键词:osteoporosis naringin network pharmacology signaling pathway |
| 基金项目:国家自然科学基金项目（81904225，81774338，81674000）；中华中医药学会（2019-2021年度）青年人才托举工程项目（CACM-2019- C08）；广东省自然科学基金项目（2018A030310615）；广东省教育厅基础研究及应用基础研究项目（2018KZDXM021, 2018KTSCX041）；广东省中医药局面上项目（20191107）；广州中医药大学第一附属医院创新强院项目（2017QN08，2017TD08）；广州中医药大学青年科研培育项目（2019QNPY04）；广州中医药大学学科研究重大项目，广州中医药大学中医骨伤学科老年脊柱伤病方向学术带头人项目；广东省医学科研基金项目（A2021320）；广东省高等学校珠江学者岗位计划资助项目（2018） |
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| 中文摘要: |
| 目的 基于网络药理学探讨柚皮苷治疗骨质疏松症（osteoporosis，OP）的潜在机制。方法 根据TCMSP数据库、Swiss Target Prediction数据库和Uniprot数据库得到柚皮苷的结构及其靶点，利用GeneCards、OMIM数据库获取OP相关靶点，将交集靶点输入到STRING 数据库以获取蛋白互作（protein-protein interaction，PPI）信息，借助Cytoscape 3.7.2软件构建PPI 网络、“柚皮苷-交集靶标-骨质疏松”网络，并使用Cytoscape 3.7.2 软件、R软件进行GO功能富集分析及KEGG通路富集分析。结果 筛选得到柚皮苷105个作用靶标，其中与OP相关靶标44个，GO功能富集分析获得607个结果（P＜0.05），KEGG 通路富集分析获得43个结果，其中相关信号通路有11条（P＜0.05），涉及 p53、IL-17、NF-κ B、Estrogen、Prolactin等信号通路。结论 柚皮苷可能通过多靶点、多通路调节炎症反应、细胞周期和激素代谢来治疗OP。 |
| 英文摘要: |
| Objective To investigate the underlying mechanism of naringin in the treatment of Osteoporosis (OP) on the basis of network pharmacology. Methods The structure of naringin and its targets were obtained through TCMSP database, Swiss Target Prediction database and Uniprot database. The OP-related targets were retrieved through GeneCards database and OMIM database. Protein-protein interaction (PPI) information were obtained with the intersection targets input into STRING database. Cytoscape3.7.2 software was used to construct PPI network and "naringin-intersection target-osteoporosis" network. Using Cytoscape3.7.2 software and R software, GO function enrichment analysis and KEGG pathway enrichment analysis were conducted. Results 105 targets of naringin were selected, including 44 targets correlated with OP. There were 607 results gained through GO function enrichment analysis (P < 0.05), and 43 results through KEGG pathway enrichment analysis, including 11 relevant signaling pathways (P < 0.05), which involved p53, IL-17, NF kappa B, Estrogen, Prolactin and other signaling pathways. Conclusion By regulating inflammatory response, cell cycle and hormone metabolism, naringin may treat OP through numerous targets and pathways. |
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