| Objective To explore the internal molecular mechanism of Bushen Huoxue Decoction "from deficiency to stasis" in the treatment of osteoporosis (OP) by network pharmacology and molecular docking technology. Methods The related chemical components and targets of Bushenhuoxue Decoction were collected from tcmsp, Batman TCM, chemical database, tcmid database and HowNet, and then the target was annotated with gene; the disease-related targets were retrieved from geencards and OMIM databases to obtain the corresponding targets; and then, used Cytoscape 3.7.2 software to visualize the corresponding relationship between drug components, diseases and targets to construct a "traditional Chinese medicine component target disease" network, and obtained the intersection target data from string database to construct protein interaction network; useed R language software to analyze gene enrichment of go and KEGG; finally, molecular docking was carried out for core compounds and targets. Results A total of 171 active compounds of Bushen Huoxue Decoction were obtained, 11 core components were screened out, 166 corresponding targets were predicted; 1335 OP related targets, 54 genes were intersected; 13 core target proteins were screened by PPI protein network; 279 and 63 enrichment results were obtained by go and KEGG enrichment analysis; molecular docking results showed that the core compounds stigmasterol and mapk1, PPARG and β-sitosterol had good binding properties with PPARG in the treatment of osteoporosis. Conclusion Bushen Huoxue Decoction plays an important role in nutritional support, immune regulation, anti-inflammatory, damage repair and other aspects. Through network pharmacology and molecular docking, it explains the internal molecular mechanism of "from deficiency to blood stasis" in the treatment of osteoporosis. |