| Objective To explore the pathological mechanism of postmenopausal osteoporosis of kidney-yin deficiency and the therapeutic target of Rehmanniae by bio-information data analysis. Methods The differentially expressed genes in postmenopausal osteoporosis patients with yin deficiency were analysed in GEO database. GeneCards and OMIM database were used to predict osteoporosis-related genes, and R software was used to map a Venn diagram of yin deficiency and osteoporosis matching. Use the String database online platform for protein interaction network construction. Molecular docking predicts the targets of Rehmanniae in the treatment of osteoporosis of yin deficiency. Use the DAVID database to annotate biological processes and pathways. Results Through analysis, 1272 differentially expressed genes, 662 osteoporosis related targets, and 45 yin deficiency osteoporosis related targets were obtained, mainly involving biological processes such as apoptosis regulation, estrogen response, and skeletal system development. It was regulated by PI3K-Akt, Wnt signaling pathway, MAPK signaling pathway, Ovarian steroidogenesis and some other signaling pathways. The two active ingredients of Rehmanniae could act on the hub proteins IGF1, VEGFA, etc. Conclusion Rehmanniae can target hub proteins such as IGF1 and VEGFA, regulate the apoptosis and estrogen response via PI3K-Akt, MAPK signaling pathway and some other signaling pathways, so as to achieve the effect of preventing and treating yin deficiency osteoporosis. |