| Objective To explore the relationship between LncRNA and osteoporosis through gene co-expression weight network analysis (WGCNA). Methods Collected 80 samples of GSE56815 chip in GEO database, obtained 127 LncRNA by re-annotation, used WGCNA to analyze the clustering analysis of the expression data, and analyzed the correlation with osteoporosis. Find the target genes of the most relevant modules and draw a network relationship diagram, and then perform GO and KEGG enrichment analysis. Results A total of 9 LncRNA modules were detected. The key module was the turquoise module containing 29 target genes, and 204 target genes were obtained. After drawing the network diagram, the hub LncRNA was ACVR2B-AS1, LINC01278, SND1-IT1, C22orf24, ZNF213 -AS1, WT1-AS, PLAC4, RHPN1-AS1 and LINC01140. The GO function was mainly focused on the transmission of information between cells; the cell function was concentrated in the nucleus, cell membrane and mitochondria, and was involved in the regulation of osteoblast differentiation. KEGG was mainly enriched in GnRH, Notch, neurotrophic and calcium ion signaling pathways. Conclusion The WGCNA method was used to obtain modules related to osteoporosis traits, to identify biologically meaningful gene modules, to obtain core LncRNA, and to perform enrichment analysis by searching for potential target genes. These results provide new insights into the study of LncRNA in the pathophysiology of osteoporosis. |