| Objective To study the effect of resveratrol (R) on osteoporosis in ovariectomized rats. Methods Forty 6-month-old Wistar female rats were randomly divided into sham group (Sham), ovariectomy group (OVX), icariin group (I), and resveratrol group (R). Rats in group I received 25 mg/(kg·d) of icariin by gavage. Rats in group R received 8.4 mg/(kg·d) resveratrol by gavage. Rats in Sham and OVX group received equal volume of distilled water. According to body weight, the gavage dose was adjusted every 2 weeks. The rats were killed 8 weeks later when there was a significant difference in bone mineral density (BMD) between the groups. The body weight of the rats was counted and the organ index of the rats was calculated. The in vitro BMD, bone biomechanics, serum biochemical indicators, bone tissue morphology, and static parameters related to trabecular bone were measured and analyzed. Results There was no statistical difference in the body weight of rats between each group, and there was no statistical difference in the index of other organs except for the uterine coefficient (P>0.05). The uterine index of OVX group was significantly lower than that of sham group (P<0.05). The uterine index of group I and R was significantly higher than that of OVX group (P<0.05). Compared with those in OVX group, BMD, maximum load, elastic modulus, and bone formation index OC, TB. N, and BV/TV in group I and R increased significantly (P<0.05), while the content of bone resorption indexes Tracp5b and TB.SP decreased significantly (P<0.01). The observation results of bone morphology showed that the trabecular reticular structure in group I and group R increased in density, and the number of trabeculae increased significantly. The average value of each index in Group R was slightly lower than that in group I, with no significant difference (P>0.05). Conclusion Resveratrol may play an anti-osteoporotic effect by increasing BMD, improving bone strength, promoting bone formation, inhibiting bone resorption, and improving bone quality. |