| Objective To identify the the molecular mechanism of eucommiae in the treatment of osteoporotic fracture based on network pharmacology. Methods The chemical components and targets for the therapeutic actions of eucommiae were screened using the TCMSP database and the GeneCards database. The intersection of therapeutic target of eucommiae and the target of osteoporosis disease was obtained with R software. Cytoscape was used to visualize the multiple components-multiple target-multiple pathways-multiple disease network of eucommiae. The regulatory network of TCM-component-disease-target was constructed. The core target was screened by constructing the protein-protein interaction (PPI) network and bar graph of the TCM-disease target. Pathway enrichment analysis was performed using the GO function enrichment analysis and KEGG pathway enrichment analysis. Results There were 19 active components in the treatment of osteoporotic fracture and 59 traditional Chinese medicine-disease targets. The enrichment of GO function involved in eucommiae-OPF target included nuclear receptor activity, transcription factor activity, steroid receptor activity and so on. The results of enrichment analysis of KEGG signal pathways involved in eucommiae-osteoporotic fracture target mainly included apoptosis signal pathway, endocrine resistance, p53 signal pathway, MAPK signal pathway, NF-kappaB signal pathway, and PI3K-Akt signal pathway. Conclusion The mechanism of eucommie in the treatment of osteoporotic fracture is that multiple active components in eucommie regulate several related genes in the process of osteoporotic fracture healing, and then mediate multiple signal pathways in the process of fracture healing. Through the integration of information reported in related literature, it is found that these pathways mainly include apoptosis, PI3K-Akt, MAPK, p53, NF-kappaB, and so on. These pathways regulate the homeostasis of bone metabolism directly or indirectly, and finally achieve the purpose of treating osteoporotic fractures. |