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| 骨质疏松症与溃疡性结肠炎共病联系的生物信息学分析 |
| Analysis of the interaction relationship between osteoporosis and ulcerative colitis based on bioinformatics |
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| DOI：10.3969/j.issn.1006-7108.2021.07.003 |
| 中文关键词: 骨质疏松 溃疡性结肠炎 生物信息学 微小核糖核苷 |
| 英文关键词:osteoporosis ulcerative colitis bioinformatics microRNA |
| 基金项目:国家自然科学基金（81873320，81973878）；江苏省自然科学基金（BK20180167）；江苏省中医药领军人才项目（SLJ0218） |
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| 中文摘要: |
| 目的 借助生物信息学理论方法探索骨质疏松症与溃疡性结肠炎之间的共病联系。方法 从GEO数据库检索获取骨质疏松症与溃疡性结肠炎基因芯片数据，筛选出差异miRNA，通过取交集的方式获取联系两疾病的共病miRNA；使用miRWalk2.0数据库预测共病miRNA的靶标基因，STRING V11进行靶标基因的蛋白互作分析，Cytoscape构建靶标基因蛋白互作网络；使用KOBAS3.0进行miRNA靶标基因的基因本体论与信号通路富集分析。结果 从GEO数据库检索获得骨质疏松症miRNA芯片GSE93883与溃疡性结肠炎miRNA芯片GSE63806，取交集后获得1个共病联系miRNA，在miRWalk2.0预测获得26个靶标基因；靶标基因间构成复杂蛋白互作网络，并从中发现3个子网络；靶标基因主要富集于调控细胞增殖分化活动、嘌呤代谢、咖啡因代谢等生物学过程及相关信号通路。结论 骨质疏松症与溃疡性结肠炎存在共同miRNA，共病miRNA可能通过调控靶标基因及其参与的下游信号通路与生物学过程联系骨质疏松症与溃疡性结肠炎，共病miRNA的发现可能有助于理解上述疾病之间的内在联系，成为新的疾病治疗靶点。 |
| 英文摘要: |
| Objective To investigate the interaction relationship between osteoporosis and ulcerative colitis using bioinformatics method. Methods The expression profiling data of osteoporosis and ulcerative colitis were collected from Gene Expression Omnibus database. The differentially expressed miRNAs of osteoporosis and ulcerative colitis were selected. The miRWalk2.0 was used for predicting targets of the selected DEmiRNAs. STRING V11 were used to visualize the protein-protein interaction of the targets of DEmiRNAs. Gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis were performed for the targets using KOBAS3.0. Results Only one DEmiRNA correlated to both osteoporosis and ulcerative colitis was selected for further analysis. Twenty-six targets were predicted for the selected DEmiRNA by miRWalk2.0 and 3 sub networks were found from the protein-protein interaction network of targets. The main enrichment of targets was in cellular process, purine metabolism, and caffeine metabolism. Conclusion We found out one miRNA that is correlated to both osteoporosis and ulcerative colitis. This miRNA may play an important role in regulating these two diseases and be a potential therapeutic target for osteoporosis and ulcerative colitis. |
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